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Product Name phospho-Histone H3 (Ser10) Chinese Name 磷酸化组蛋白H3单克隆抗体 Alias Histone H3 (phospho S10); Phospho-Histone H3(Ser10); Histone H3(Phospho-Ser10); Histone H3(p-Ser10); Histone H3(Phospho-S10); H3S10ph; H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3.1; H3/d; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C7; H3C8; H3FD; Product Type Phosphorylated anti Research Area Chromatin and nuclear signals Cell type markers Epigenetics Immunogen Species Mouse Clonality Monoclonal React Species Human, (predicted: Mouse, Rat, Pig, Cow, Rabbit, Firefly, ) Applications WB=1:1000-2000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 15kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser10: RK(p-S)TG Lsotype IgG Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
SWISS:
P68431
Gene ID:
8350
Database links:Entrez Gene: 8350 Human
Entrez Gene: 319152 Mouse
SwissProt: P68431 Human
SwissProt: P68433 Mouse
Product Picture Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L
(HRP)
Lysate: (-) HeLa, (+) HeLa+ Nocodazole
(100ng/ml, 18hr) + Calyculin A (100nM, 1hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 17 kDa
Observed MW: 17 kDa
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