Product Name	Histone H2B (Acetyl K11)
Chinese Name	乙酰化组蛋白H2B (Lys120)单克隆抗体
Alias	H2BK11ac; Histone H2B (Acetyl Lys11); Acetyl Histone H2B (K12); Histone H2B/HIST1H2BD; H2B.1; H2B.1 B; H2B.b; H2B.c; H2B.d; H2B.e; H2B.f; H2B.j; H2B.q; H2BFB; H2BFC; H2BFD; H2BFE; H2BFF; H2BFJ; H2BFO; H2BFQ; H2BFS; HIRIP2; HIST1H2BB; HIST1H2BD; HIST1H2BH; HIST1H2BL; HIST1H2BM; HIST1H2BN; HIST2H2BE; Histone H2B; Histone H2B type 1 B; Histone H2B type 1 D; Histone H2B type 1 H; Histone H2B type 1 L; Histone H2B type 1 M; Histone H2B type 1 N; Histone H2B type 2 E; Histone protein; H2B GL105; H2B histone family member O; H2B histone family member S; histone H2B type 1-B; H2B1B_HUMAN.
Product Type	Acetylated anti
Research Area	Epigenetics
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	H2C1
React Species	Human, Mouse,  (predicted: Rat, )
Applications	WB=1:500-2000 IP=1:20-100  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	14kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Histone H2B (Acetyl K11)
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]. SWISS: P33778 Gene ID: 3018
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:500 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: Mouse liver, Mouse spleen Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14 kDa Observed MW: 14 kDa Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 14 kDa Observed MW: 14 kDa IP of HeLa+Sodium butyrate (30mM, 4hr) cells extracts IP ab incubation condition: SLM-60062M , 4°C overnight, 1:25, 1:100 dilution WB primary ab incubation condition: SLM-60062M, room temperature 2h, 1:2000 dilution Secondary ab: Anti-Mouse IgG for IP (HRP) Blocking buffer and concentration: 5% NFDM/TBST Diluting buffer and concentration: 5% NFDM/TBST Lane 1: 5% Input Lane 2: IP with SLM-60062M (1:25) Lane 3: IP with SLM-60062M (1:100) Observed MW: 14 kDa Exposure time: 60 s

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