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Product Name Arginase 1 Chinese Name 精氨酸酶1Recombinant rabbit monoclonal anti Alias liver Arginase; ARG 1; ARG1; ARGI1_HUMAN; Arginase1; Arginase liver; Arginase type I; Arginase I; ArginaseI; Arginase-1; Arginase1; Liver type arginase; Liver-type arginase; Type I arginase. Research Area Tumour Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Monoclonal React Species Human, Applications WB=1:500-2000 IHC-P=1:200-1:1000 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 35kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Arginase 1 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Arginase I which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea . The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster. Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location. Specifically, arginase II localizes to the mitochondria. Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver. The human arginase II gene, which maps to chromosome 14q24.1-q24.3, encodes a 354 amino acid protein. In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence.
Subunit:
Homotrimer.
Subcellular Location:
Cytoplasm.
DISEASE:
Defects in ARG1 are the cause of argininemia (ARGIN) ; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
Similarity:
Belongs to the arginase family.
SWISS:
P05089
Gene ID:
383
Database links:
Entrez Gene: 383 Human
Entrez Gene: 11846 Mouse
Entrez Gene: 100008814 Rabbit
Omim: 608313 Human
SwissProt: P05089 Human
SwissProt: Q61176 Mouse
SwissProt: Q95KM0 Rabbit
Unigene: 440934 Human
Unigene: 154144 Mouse
Unigene: 9857 Rat
Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: Human liver
Protein loading quantity: 20 μg
Exposure time: 1 s
Predicted MW: 35 kDa
Observed MW: 37 kDa
Tissue: Human liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60019R
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Arginase 1) Monoclonal Antibody, Unconjugated (SLM-60019R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human liver ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Arginase 1) Monoclonal Antibody, Unconjugated (SLM-60019R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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