Product Name	Glypican 3
Chinese Name	磷脂酰基醇蛋白聚糖-3Recombinant rabbit monoclonal anti
Alias	GPC3; glypican3; glypican-3; Intestinal protein OCI-5; GTR2-2; MXR7.Intestinal protein OCI-5; GPC3_HUMAN.
Research Area	Tumour  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Monoclonal
React Species	Human,
Applications	WB=1:500-2000 Flow-Cyt=1:20-100 ICC=1:20-100 IF=1:20-100  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	65kDa
Cellular localization	The cell membrane Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Glypican 3
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009] Function: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Subcellular Location: Cell membrane and Secreted, extracellular space. Tissue Specificity: Highly expressed in lung, liver and kidney. DISEASE: Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Similarity: Belongs to the glypican family. SWISS: P51654 Gene ID: 2719 Database links: Entrez Gene: 2719 Human Entrez Gene: 14734 Mouse Entrez Gene: 25236 Rat Omim: 300037 Human SwissProt: P51654 Human SwissProt: Q8CFZ4 Mouse SwissProt: P13265 Rat Unigene: 644108 Human Unigene: 22515 Mouse Unigene: 9717 Rat GPC-3磷脂酰基醇蛋白聚糖-3是一种硫酸类肝素蛋白多糖，GPC-3在不同的组织中具有不同的生物学功能，甚至起着完全不同的作用，可能与Tumour组织类型、发病机制等密切相关．GPC3在绝大多数HCC(肝细胞癌)患者有表达。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: HepG2 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 66 kDa Observed MW: 66 kDa Cell line: HepG2 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60006R Cell line: HepG2 Fixative: 4% Paraformaldehyde Permeabilization: 90% methanol Primary ab dilution: 1:100 Secondary ab: Goat anti Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for SLM-60006R

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