Product Name	Carbonic Anhydrase I
Chinese Name	碳酸酐酶1Recombinant rabbit monoclonal anti
Alias	Carbonic Anhydrase-I; Carbonic Anhydrase 1; Carbonic anhydrase1; Carbonic anhydrase-1; JW0122; CA 1; CA1; CA I; CA-1; CAI; CAN; Car 1; Car1; Carbonate dehydratase I; Carbonic anhydrase 1; Carbonic anhydrase B; Carbonic dehydratase; ECK0125; yadF; CAH1_HUMAN.
Research Area	Cardiovascular  Cell biology  immunology  Signal transduction
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	2H4
React Species	Mouse, Rat,  (predicted: Human, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	29kDa
Cellular localization	The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human Carbonic Anhydrase I: 1-200/261
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq, Sep 2009] Function: Reversible hydration of carbon dioxide. Subcellular Location: Cytoplasm. Similarity: Belongs to the alpha-carbonic anhydrase family. SWISS: P00915 Gene ID: 759 Database links: Entrez Gene: 759 Human Entrez Gene: 12346 Mouse Entrez Gene: 310218 Rat Omim: 114800 Human SwissProt: P00915 Human SwissProt: P13634 Mouse SwissProt: B0BNN3 Rat Unigene: 23118 Human Unigene: 273195 Mouse Unigene: 6854 Rat
Product Picture	Sample: Lane 1: Large intestine (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Lane 3: Stomach (Mouse) Lysate at 40 ug Lane 4: Bone (Mouse) Lysate at 40 ug Lane 5: Spleen (Mouse) Lysate at 40 ug Lane 6: Small intestine (Mouse) Lysate at 40 ug Lane 7: Lung (Mouse) Lysate at 40 ug Lane 8: Cerebrum (Mouse) Lysate at 40 ug Lane 9: Large intestine (Rat) Lysate at 40 ug Lane 10: Lung (Rat) Lysate at 40 ug Lane 11: Stomach (Rat) Lysate at 40 ug Lane 12: Bone (Rat) Lysate at 40 ug Lane 5: Spleen (Rat) Lysate at 40 ug Primary: Anti-Carbonic Anhydrase I (SLM-54409R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 27 kD

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