Product Name	PICK1
Chinese Name	蛋白激酶Cα相互作用蛋白1Recombinant rabbit monoclonal anti
Alias	PICK 1; Pick1; PICK1_HUMAN; PRKCA binding protein; PRKCA-binding protein; Prkcabp; Protein interacting with C kinase 1; Protein interacting with PRKCA; Protein interacting with PRKCA 1; Protein kinase C alpha binding protein; Protein kinase C-alpha-binding protein; Protein that interacts with C kinase 1.
Research Area	Tumour  Cell biology  immunology  Neurobiology  Signal transduction  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	4G1
React Species	Human,  (predicted: Mouse, Rat, )
Applications	WB=1:500-2000 IP=1:10-50 Flow-Cyt=1:50  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	46kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PICK1
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel. Function: Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel. Subunit: Monomer and homodimer. Subcellular Location: Cytoplasm, perinuclear region. Cell junction, synapse. Note=Also present at excitatory synapses. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors. Similarity: Contains 1 AH domain. Contains 1 PDZ (DHR) domain. SWISS: Q9NRD5 Gene ID: 9463 Database links: Entrez Gene: 9463 Human Entrez Gene: 18693 Mouse Entrez Gene: 84591 Rat Omim: 605926 Human SwissProt: Q9NRD5 Human SwissProt: Q62083 Mouse SwissProt: Q9EP80 Rat Unigene: 180871 Human
Product Picture	Sample: Lane 1: SK-Br-3 cell lysate Lane 2: 293 cell lysate Primary: Anti-PICK1 (SLM-54393R) at 1:500 dilution Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution Predicted band size: 46 kD Observed band size: 52 kD Blank control:293T. Primary Antibody (green line): Rabbit Anti-PICK1 antibody (bms-54393R) Dilution: 1:100 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1:1000. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Totalgoods,subtotals:￥Checkout