Rabbit Anti-CXCR5 antibody
BLR 1; BLR1; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1 GTP binding protein; C X C chemokine receptor type 5; C-X-C chemokine receptor type 5; CD 185; CD185; CD185 antigen; Chemokine C X C motif receptor 5; Chemokine CXC motif receptor 5; Ch
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Product Name CXCR5 Chinese Name 细胞表面Chemokine受体5（CD185）Recombinant rabbit monoclonal anti Alias BLR 1; BLR1; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1 GTP binding protein; C X C chemokine receptor type 5; C-X-C chemokine receptor type 5; CD 185; CD185; CD185 antigen; Chemokine C X C motif receptor 5; Chemokine CXC motif receptor 5; Chemokine receptor 5; CXC chemokine receptor type 5; CXC R5; CXC-R5; CXCR 5; CXCR-5; CXCR5_HUMAN; MDR 15; MDR-15; MDR15; MGC117347; Monocyte derived receptor 15; Monocyte-derived receptor 15. literatures Research Area Tumour Cell biology immunology Signal transduction Chemokine G protein-coupled receptor b-lymphocyte G protein signal Immunogen Species Rabbit Clonality Monoclonal Clone NO. 3B7 React Species Human, Mouse, Rat, Applications WB=1:500-1000 IHC-P=1:50-200 IHC-F=1:50-200 （Paraffin sections need antigen repair）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 45kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen Recombinant human CXCR5 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
Function:
Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Tissue Specificity:
Expression in mature B-cells and Burkitt lymphoma cells.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
P32302
Gene ID:
643
Database links:Entrez Gene: 643 Human
Entrez Gene: 12145 Mouse
Omim: 601613 Human
SwissProt: P32302 Human
SwissProt: Q04683 Mouse
Unigene: 113916 Human
Unigene: 490637 Mouse
Unigene: 6246 Mouse
Unigene: 120732 Rat
Unigene: 127121 Rat
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Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: THP-1 (Human) Cell Lysate at 30 ug
Lane 3: U937 (Human) Cell Lysate at 30 ug
Lane 4: Molt-4 (Human) Cell Lysate at 30 ug
Lane 5: K562 (Human) Cell Lysate at 30 ug
Lane 6: A673 (Human) Cell Lysate at 30 ug
Primary: Anti-CXCR5 (SLM-54319R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 45 kD
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CXCR5) Monoclonal Antibody, Unconjugated (SLM-54319R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CXCR5) Monoclonal Antibody, Unconjugated (SLM-54319R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CXCR5) Monoclonal Antibody, Unconjugated (SLM-54319R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CXCR5) Monoclonal Antibody, Unconjugated (SLM-54319R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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