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Product Name MHC class I Chinese Name 组织相容性复合体蛋白1Recombinant rabbit monoclonal anti Alias MHC-1; MHC1; MHC 1; A 28; A 9; Aw 24; Aw 68; CLASS I HISTOCOMPATIBILITY ANTIGEN; H2 K1; H2K; HLA A; HLAA; HLA-A; HLA class I histocompatibility antigen A 1 alpha chain; HLA class I histocompatibility antigen A 11 alpha chain; HLA class I histocompatibility antigen A 2 alpha chain; HLA class I histocompatibility antigen A 24 alpha chain; HLA class I histocompatibility antigen A 3 alpha chain; HLA class I histocompatibility antigen A 30 alpha chain; HLA class I histocompatibility antigen A 32 alpha chain; HLA class I histocompatibility antigen A 68 alpha chain; Major histocompatibility complex class I A; MHC class I heavy chain H2 K; 1A24_HUMAN. Research Area Tumour Cell biology immunology Signal transduction transcriptional regulatory factor Cell differentiation Immunogen Species Rabbit Clonality Monoclonal Clone NO. 10G4 React Species Human, Applications WB=1:500-2000 IHC-P=1:100 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 40kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MHC class I Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
Function:
Involved in the presentation of foreign antigens to the immune system.
Subunit:
Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein (By similarity).
Subcellular Location:
Cell Membrane; Type I membrane protein.
Post-translational modifications:
Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).
Similarity:
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
SWISS:
P05534
Gene ID:
3105
Database links:Entrez Gene: 3105 Human
Omim: 142800 Human
SwissProt: P30443 Human
Unigene: 181244 Human
Product Picture Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human U266 cell lysates
Primary: Anti-MHC class I (SLM-52827R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 42 kDa
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MHC class I) Monoclonal Antibody, Unconjugated (SLM-52827R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MHC class I) Monoclonal Antibody, Unconjugated (SLM-52827R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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