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Product Name Cyclophilin B(Loading Control) Chinese Name 亲环蛋白(亲环素)PPIB(内参)Recombinant rabbit monoclonal anti Alias Cphn 2; Cphn2; CyP 20b; CYP S1; CYP-S1; CYPB; MGC14109; MGC2224; peptidyl prolyl cis trans isomerase B; Peptidyl prolyl cis trans isomerase B precursor; Peptidyl-prolyl cis-trans isomerase B precursor; Peptidylprolyl isomerase B; PPIase; PPIB; Rotamase; S cyclophilin; SCYLP; PPIB_HUMAN. Product Type Internal reference anti Recombinant rabbit monoclonal anti Research Area Tumour Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Monoclonal Clone NO. 4A10 React Species Human, Mouse, Rat, Applications WB=1:500-1000 IHC-P=1:400-800 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 20kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen Recombinant human Cyclophilin B: C terminus Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The cyclophilins are a conserved class of proteins that bind the immunosuppressive drug cyclosporin A (CsA) with high affinity. CsA blocks helper T-cell activation at a step between T-cell receptor stimulation and the transcriptional activation of cytokine genes. Cyclophilins from many species possess peptidyl-prolyl cis-trans isomerase (PPIase) activity that is blocked by CsA and therefore may be relevant in CsA-mediated immunosuppression.
Function:
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Subcellular Location:
Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
DISEASE:
Defects in PPIB are the cause of osteogenesis imperfect ype 9 (OI9) [MIM:259440]. OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients.
Similarity:
Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
Contains 1 PPIase cyclophilin-type domain.
SWISS:
P23284
Gene ID:
5479
Database links:Entrez Gene: 5479 Human
Entrez Gene: 19035 Mouse
Omim: 123841 Human
SwissProt: P23284 Human
SwissProt: P24369 Mouse
Unigene: 434937 Human
Unigene: 335249 Mouse
Unigene: 1893 Rat
Product Picture Sample:
Lane 1: Placenta (Rat) Lysate at 40 ug Lane 2: Liver (Mouse) Lysate at 40 ug Lane 3: Kidney (Mouse) Lysate at 40 ug Lane 4: Cerebrum (Rat) Lysate at 40 ug Lane 5: Liver (Rat) Lysate at 40 ug Lane 6: Kidney (Rat) Lysate at 40 ug Lane 7: Hela (Human) Cell Lysate at 30 ug Lane 8: A431 (Human) Cell Lysate at 30 ug Lane 9: HepG2 (Human) Cell Lysate at 30 ug Lane 10: Huvec (Human) Cell Lysate at 30 ug Lane 11: U937 (Human) Cell Lysate at 30 ug Primary: Anti- Cyclophilin B (SLM-52473R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 18 kD
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: Thyroid gland (Rat) Lysate at 40 ug
Lane 5: Thyroid gland (Mouse) Lysate at 40 ug
Lane 6: Placenta (Rat) Lysate at 40 ug
Lane 7: Placenta (Mouse) Lysate at 40 ug
Lane 8: Cerebrum (Rat) Lysate at 40 ug
Lane 9: Cerebrum (Mouse) Lysate at 40 ug
Lane 10: Liver (Rat) Lysate at 40 ug
Lane 11: Liver (Mouse) Lysate at 40 ug
Lane 12: Kidney (Rat) Lysate at 40 ug
Lane 13: Kidney (Mouse) Lysate at 40 ug
Lane 14: Huvec (Human) Cell Lysate at 30 ug
Primary: Anti-Cyclophilin B (SLM-52473R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclophilin B(Loading Control)) Monoclonal Antibody, Unconjugated (SLM-52473R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclophilin B(Loading Control)) Monoclonal Antibody, Unconjugated (SLM-52473R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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