Product Name	Tubulin beta-III
Chinese Name	微管蛋白β3Recombinant rabbit monoclonal anti
Alias	Neuron specific beta III Tubulin; beta 4; MC1R; TBB3_HUMAN; TUBB 3; TUBB 4; TUBB3; TUBB4; Tubulin beta 3 chain; Tubulin beta 4; Tubulin beta III; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; Beta tubulin III; Neuron specific beta III Tubulin.
literatures	Specific References  (2)     |     SLM-52323R has been referenced in 2 publications. [IF=6.064] Le Wang. et al. Enteric nervous system damage caused by abnormal intestinal butyrate metabolism may lead to functional constipation. FRONT MICROBIOL. 2023; 14: 1117905  ICC ;  Mouse.   PubMed:37228368 [IF=5.195] Lu Hong. et al. Investigation of Naoluoxintong on the neural stem cells by facilitating proliferation and differentiation in vitro and on protecting neurons by up-regulating the expression of nestin in MCAO rats. J ETHNOPHARMACOL. 2022 Sep;:115684  WB ;  Rat.   PubMed:36058480
Research Area	Cell biology  Neurobiology  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	3B7
React Species	Human, Mouse, Rat,
Applications	WB=1:1000-2000 IHC-P=1:50-200 IHC-F=1:50-200 Flow-Cyt=1:50 ICC=1:50 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Tubulin beta-III
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Subcellular Location: Cytoplasm, cytoskeleton. Tissue Specificity: Expression is primarily restricted to central and peripheral nervous system. Post-translational modifications: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. DISEASE: Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy. Similarity: Belongs to the tubulin family. SWISS: Q13509 Gene ID: 10381 Database links: Entrez Gene: 10381 Human Entrez Gene: 431043 Chicken Entrez Gene: 22152 Mouse Entrez Gene: 246118 Rat Omim: 602661 Human SwissProt: Q2T9S0 Cow SwissProt: Q13509 Human SwissProt: Q9ERD7 Mouse SwissProt: Q4QRB4 Rat Unigene: 511743 Human Unigene: 40068 Mouse Unigene: 43958 Rat
Product Picture	Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Rat Cerebellum tissue lysates Lane 5: Human SH-SY5Y cell lysates Lane 6: Human U251 cell lysates Lane 7: Human U87MG cell lysates Primary: Anti-Tubulin beta-III (SLM-52323R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa Sample: Lane 1: PC-12 cell lysate Lane 2: Hela cell lysate Lane 3: SH-SY5Y cell lysate Primary: Anti-Tubulin beta-III (SLM-52323R) at 1:500 dilution Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution Predicted band size: 50 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (SLM-52323R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Tubulin beta-III (3B7)) Monoclonal Antibody, Unconjugated (SLM-52323R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. SHG-44 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Tubulin beta-III (3B7)) Monoclonal Antibody, Unconjugated (SLM-52323R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. Blank control:N2A. Primary Antibody (green line): Rabbit Anti-TUBB3 antibody (SLM-52323R) Dilution: 1:50; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1:1000. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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