Product Name	CD9
Chinese Name	CD9Recombinant rabbit monoclonal anti
Alias	Tetraspanin 29; 5H9; 5H9 antigen; Antigen defined by monoclonal; 602 29; Antigen defined by monoclonal; 60229; BA 2; BA2; BTCC 1; BTCC1; CD 9; CD9; CD9 antigen; CD9 antigen p24; CD9 molecule; CD9_HUMAN; Cell growth inhibiting gene 2 protein; Cell growth-inhibiting gene 2 protein; DRAP 27; DRAP27; GIG 2; GIG2; Growth inhibiting gene 2 protein; Leukocyte antigen MIC3; MIC 3; MIC3; Motility related protein; Motility-related protein; MRP 1; MRP-1; MRP1; p24; P24; p24 antigen; Tetraspanin 29; Tetraspanin-29; Tetraspanin29; Tspan 29; Tspan-29; Tspan29.
Research Area	Tumour  Cell biology  immunology  Developmental biology  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	4C2
React Species	Human, Mouse,  (predicted: Rat, )
Applications	WB=1:500-1000 ELISA=1:5000-10000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:100 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	25kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human CD9: 111-196/228 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve. Function: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Subunit: Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expressed by a variety of hematopoietic and epithelial cells. Post-translational modifications: Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. Similarity: Belongs to the tetraspanin (TM4SF) family. SWISS: P21926 Gene ID: 928 Database links: Entrez Gene: 928 Human Entrez Gene: 12527 Mouse Entrez Gene: 24936 Rat Omim: 143030 Human SwissProt: P21926 Human SwissProt: P40240 Mouse SwissProt: P40241 Rat Unigene: 114286 Human Unigene: 210676 Mouse Unigene: 404614 Mouse Unigene: 2091 Rat CD9是一种普遍存在于The cell membrane表面属于四跨膜超蛋白家族的细胞表面glycoprotein, 参与质膜的融合过程，在The cell membrane生物学中发挥重要作用。 近年来经研究发现，CD9与多种Tumour转移有关，故称Tumour转移抑制基因亦称抗Tumour转移基因。是一个新的Tumour转移抑制基因膜运动相关蛋白-1(CD9/MRP-1)，其分子功能还有待于进一步研究。CD9主要在早B细胞、活化Tlymphocyte、嗜酸性粒细胞、嗜碱性粒细胞和血小板表达。
Product Picture	Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug Lane 2: Testis (Mouse) Lysate at 40 ug Lane 3: Heart (Mouse) Lysate at 40 ug Lane 4: Cerebrum (Mouse) Lysate at 40 ug Lane 5: Huvec (Human) Cell Lysate at 30 ug Lane 6: A431 (Human) Cell Lysate at 30 ug Lane 7: SW480 (Human) Cell Lysate at 30 ug Primary: Anti-CD9 (SLM-52264R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 24 kD Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (SLM-52264R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (SLM-52264R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (SLM-52264R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (SLM-52264R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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