Product Name	SIRT2
Chinese Name	沉默调节相关蛋白2Recombinant rabbit monoclonal anti
Alias	FLJ35621; FLJ37491; NAD dependent deacetylase sirtuin 2; NAD-dependent deacetylase sirtuin-2; Regulatory protein SIR2 homolog 2; Silencing information regulator 2 like; Silent information regulator 2; Silent mating type information regulation 2; Silent mating type information regulation 2 homolog; SIR 2; SIR2; SIR2 like; SIR2 like protein 2; Sir2 related protein type 2; SIR2-like protein 2; SIR2L; SIR2L2; SIRT 2; SIRT2; SIRT2_HUMAN; Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae); Sirtuin 2; Sirtuin type 2; Sirtuin2.
Research Area	Cell biology  Signal transduction  Cyclin  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	4G12
React Species	Human, Rat,  (predicted: Mouse, )
Applications	WB=1:200-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	36kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human SIRT2 protein, around C-terminal 150aa
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene. [provided by RefSeq, Jul 2010] Function: NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Subcellular Location: Cytoplasm, cytoskeleton. Colocalizes with microtubules. Tissue Specificity: Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. Post-translational modifications: Phosphorylated at the G2/M transition of the cell cycle. Similarity: Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain. SWISS: Q8IXJ6 Gene ID: 22933 Database links: Entrez Gene: 22933 Human Omim: 604480 Human SwissProt: Q8IXJ6 Human Unigene: 466693 Human
Product Picture	Sample: Lane 1: U87MG (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Lane 3: Huvec (Human) Cell Lysate at 30 ug Lane 4: THP-1 (Human) Cell Lysate at 30 ug Primary: Anti- SIRT2 (SLM-52221R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43/37 kD Observed band size: 43/37 kD Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SIRT2) Monoclonal Antibody, Unconjugated (SLM-52221R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SIRT2) Monoclonal Antibody, Unconjugated (SLM-52221R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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