Product Name	Phospho-Glycogen synthase 1 (Ser641)
Chinese Name	磷酸化葡萄糖合成酶1Recombinant rabbit monoclonal anti
Alias	Glycogen synthase 1(S641); Glycogen synthase 1 (phospho S641); Glycogen synthase 1 (phospho Ser641); p-Glycogen synthase 1 (S641); Glycogen synthase 1 (muscle); Glycogen synthase 1; GSY; GYS; GYS1; EC 2.4.1.11; Glycogen synthase1; GYS 1; Starchsynthase muscle; UDP glucose glycogen glucosyltransferase; GYS1_HUMAN; Glycogen [starch] synthase, muscl.
Product Type	Phosphorylated anti Recombinant rabbit monoclonal anti
Research Area	Tumour  Signal transduction  transcriptional regulatory factor  Synthesis and Degradation  glycoprotein  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	10C1
React Species	Human, Mouse, Rat,
Applications	WB=1:1000-2000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	85kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human Glycogen synthase 1 around the phosphorylation site of Ser641: PA(p-S)VP
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Glycogen Synthase (GS) is a key enzyme in the regulation of glycogen metabolism. GS catalyzes the incorporation of UDP-glucose incorporation into glycogen. The activity of glycogen synthase is regulated by hormonal stimuli (insulin, catecholamines and glucagons) and non-hormonal stimuli (blood glucose level and exercise). Two main isoforms of mammalian GS are designated as muscle (glycogen synthase 1) and liver (glycogen synthase 2). Most tissues express glycogen synthase 1, whereas glycogen synthase 2 appears to be tissue-specific. The two isoforms have 70% identical amino acid sequence. Glycogen synthase can be phosphorylated by multiple kinases including glycogen synthase kinase-3 (GSK-3), mitogen-activated protein kinase-related protein kinase (DYRK), and SAPK2b/p38b which leads to its inactivation. Function: Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. Subunit: Interacts with GYG1. Post-translational modifications: Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity). Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme. DISEASE: Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work. Similarity: Belongs to the glycosyltransferase 3 family. SWISS: P13807 Gene ID: 2997 Database links: Entrez Gene: 2997 Human Entrez Gene: 14936 Mouse Entrez Gene: 690987 Rat Omim: 138570 Human SwissProt: P13807 Human SwissProt: Q8VEB0 Mouse SwissProt: Q9Z1E4 Mouse SwissProt: A2RRU1 Rat Unigene: 386225 Human Unigene: 275654 Mouse Unigene: 95278 Rat
Product Picture	SLM-52159R_BA02268251_20211015 Phospho-Glycogen synthase 1 (Ser641) (85kD) Sample: Lane 1: Human NIH/3T3 cell lysates Lane 2: Rat Skeletal muscle lysates Lane 3: Rat Heart lysates Primary: Anti-Phospho-Glycogen synthase 1 (Ser641) (SL15418R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 85 kDa Observed band size: 80 kDa Paraformaldehyde-fixed, paraffin embedded (human myocardium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (SLM-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (SLM-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (SLM-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (SLM-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (SLM-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (SLM-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (SLM-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (SLM-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (SLM-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Glycogen synthase 1 (Ser641)) Monoclonal Antibody, Unconjugated (SLM-52159R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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