Rabbit Anti-IRF3 antibody
Interferon regulatory factor 3; IRF-3; IRF 3; IRF3_HUMAN.
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Product Name IRF3 Chinese Name Interferon调节因子3Recombinant rabbit monoclonal anti Alias Interferon regulatory factor 3; IRF-3; IRF 3; IRF3_HUMAN. Research Area Tumour immunology Apoptosis transcriptional regulatory factor Immunogen Species Rabbit Clonality Monoclonal Clone NO. 4C3 React Species Human, Mouse, (predicted: Rat, ) Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200 （Paraffin sections need antigen repair）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 47kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/1ml immunogen Recombinant human IRF3 (50-150aa) Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011].
Function:
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Subunit:
Monomer. Homodimer; phosphorylation-induced. Heterodimer with IRF7. Interacts with CREBBP. May interact with MAVS. Interacts with IKBKE and TBK1. Interacts with TICAM1 and TICAM2. Interacts with rotavirus A NSP1 (via C-terminus); this interaction leads to the proteasome-dependent degradation of IRF3. Interacts with RBCK1. Interacts with TRIM21. Interacts with HERC5.
Subcellular Location:
Cytoplasm. Nucleus. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
Tissue Specificity:
Expressed constitutively in a variety of tissues.
Post-translational modifications:
Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.
Similarity:
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
SWISS:
Q14653
Gene ID:
3661
Database links:Entrez Gene: 3661 Human
Entrez Gene: 54131 Mouse
Omim: 603734 Human
SwissProt: Q14653 Human
SwissProt: P70671 Mouse
Unigene: 289052 Human
Unigene: 75254 Human
Unigene: 3960 Mouse
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Sample:
Hela (Human) Cell Lysate at 30 ug
Testis (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
A549(Human) Cell Lysate at 30 ug
Primary: Anti- IRF3 (SLM-52116R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 50-55 kD
Sample:
HepG2(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti-IRF3 (SLM-52116R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45-55 kD
Observed band size: 55 kD
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