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Product Name PURB Chinese Name 转录激活蛋白PURB单克隆抗体 Alias Pur beta; PURB_HUMAN; PURBETA; Purine rich element binding protein B; Purine-rich element-binding protein B; transcriptional activator protein Pur beta; Transcriptional activator protein Pur-beta. Research Area Chromatin and nuclear signals transcriptional regulatory factor Epigenetics Immunogen Species Mouse Clonality Monoclonal Clone NO. F4D9 React Species Human, Mouse, Applications WB=1:1000-4000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 33kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen Recombinant human PURB. Lsotype IgG1,κ Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]
Function:
Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs.
Subunit:
Homodimer, heterodimer with PURA and heterotrimer with PURA and YBX1/Y-box protein 1.
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in myocardium of heart failure patients.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Belongs to the PUR DNA-binding protein family.
SWISS:
Q96QR8
Gene ID:
5814
Database links:Entrez Gene: 5814 Human
Entrez Gene: 19291 Mouse
SwissProt: Q96QR8 Human
SwissProt: O35295 Mouse
Product Picture Sample:
Lane 1: Jurkat cell lysates
Lane 2: HeLa cell lysates
Lane 3: NCI-H460 cell lysates
Lane 4: HepG2 cell lysates
Lane 5: MCF-7 cell lysates
Lane 6: NIH/3T3 cell lysates
Primary: Anti-PURB (SLM-51738M) at 1/8000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 37 kD
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