Product Name	PLIN3
Chinese Name	PLIN3单克隆抗体
Alias	TIP47; PLIN3_HUMAN; 47 kDa MPR binding protein; Cargo selection protein TIP47; M6PRBP 1; M6PRBP1; Mannose 6 phosphate receptor binding protein 1; MGC11117; MGC2012; MPR binding protein 47 kDa; Perilipin 3; Perilipin3; Perilipin-3; Placental protein 17; PLIN3; PP17.
Research Area	Signal transduction
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	D2A3
React Species	Human,
Applications	WB=1:500-2000 Flow-Cyt=1:25  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	48kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PLIN3: 1-434/434
Lsotype	IgG2a,k
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2009] Subunit: Homooligomer. Interacts with M6PR (via the cytoplasmic domain). Interacts with IGF2R (via the cytoplasmic domain). Isoform 2 may exist as a homodimer (known as PP17C). Subcellular Location: Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Lipid droplet (Potential). Note=Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails. Similarity: Belongs to the perilipin family. SWISS: O60664 Gene ID: 10226 Database links: Entrez Gene: 10226 Human SwissProt: O60664 Human
Product Picture	Sample: Lane 1: Hela cell lysates Lane 2: K562 cell lysates Lane 3: Daudi cell lysates Primary: Anti-PLIN3 (SLM-51728M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD Blank control: HUVEC. Primary Antibody (green line): Mouse Anti-PLIN3 antibody (SLM-51728M) Dilution: 1:25; Isotype Control Antibody (blue line): Mouse IgG Secondary Antibody : Goat anti-mouse IgG-AF488 Dilution: 1:200 Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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