Product Name	HACE1
Chinese Name	E3Ubiquitin蛋白连接酶HACE1单克隆抗体
Alias	E3 ubiquitin-protein ligase HACE1; HACE 1; HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; KIAA1320; HACE1_HUMAN.
Research Area	Tumour  Cell biology  Chromatin and nuclear signals  Cyclin  Epigenetics
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	B4F6
React Species	Human,
Applications	WB=1:500-1000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	90-102kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human HACE1.
Lsotype	IgG1,k
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor. [provided by RefSeq, Mar 2016] Function: E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB. Subunit: Interacts with RARB (By similarity). Interacts with RAB1 (RAB1A, RAB1B or RAB1C), RAB4 (RAB4A or RAB4B) and RAB11 (RAB11A or RAB11B); in a GTP-dependent manner. Interacts with RAC1; in a GTP-dependent manner. Interacts with the 26S proteasomal complex through the 20S core proteasomal subunit. Subcellular Location: Cytoplasm. Endoplasmic reticulum. A significant portion localizes to the endoplasmic reticulum. Tissue Specificity: Expressed in multiple tissues including heart, brain and kidney. DISEASE: Note=Defects in HACE1 are a cause of Wilms tumor (WT). WT is a pediatric malignancy of kidney and one of the most common solid cancers in childhood. HACE1 is epigenetically down-regulated in sporadic Wilms tumor. Moreover, a t(5;6)(q21;q21) translocation that truncates HACE1 has been found in a child with bilateral, young-onset Wilms tumor. Similarity: Contains 6 ANK repeats. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. SWISS: Q8IYU2 Gene ID: 57531 Database links: Entrez Gene: 57531 Human SwissProt: Q8IYU2 Human
Product Picture	Sample: Lane 1: 293 cell lysates Lane 2: 293T/17 cell lysates Primary: Anti-HACE1 (SLM-51691M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 90-102 kD Observed band size: 97 kD

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