TEL: +86 571 56623320 EMAIL: [email protected]
Product Name HAVCR2/TIM-3 Chinese Name HAVCR2(CD366)单克隆抗体 Alias TIM-3; Tim 3; TIM3; TIMD-3; TIMD3; HAVR2_HUMAN; Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; CD366; Research Area immunology Cell Surface Molecule t-lymphocyte Immunogen Species Mouse Clonality Monoclonal Clone NO. H9F4 React Species Human, Applications WB=1:500-2000 Flow-Cyt=1:25
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 31kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen Recombinant human HAVCR2. Lsotype IgG2b,k Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
Function:
Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9.
Subcellular Location:
Membrane.
Tissue Specificity:
T-helper type 1 lymphocyte (Th1)-specific.
Post-translational modifications:
O-glycosylated with core 1 or possibly core 8 glycans.
Similarity:
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
SWISS:
Q8TDQ0
Gene ID:
84868
Database links:Entrez Gene: 84868 Human
SwissProt: Q8TDQ0 Human
Product Picture Sample:
Lane 1: HepG2 cell lysates
Lane 2: Daudi cell lysates
Lane 3: Human Kidney tissue lysates
Primary: Anti-HAVCR2 (SLM-51680M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 27 kD
Blank control:U-2 OS.
Primary Antibody (green line): Mouse Anti-HAVCR2 antibody (SLM-51680M)
Dilution: 1:25;
Isotype Control Antibody (blue line): Mouse IgG Secondary Antibody : Goat anti-mouse IgG-AF488
Dilution: 1:200 Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Scan Wechat Qrcode
Scan Whatsapp Qrcode