TEL: +86 571 56623320 EMAIL: [email protected]
Product Name Dnmt3a Chinese Name DNA甲基转移酶-3α单克隆抗体 Alias DNA (cytosine 5 ) methyltransferase 3 alpha; DNA (cytosine 5) methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaI; DNA MTase HsaIIIA; DNMT 3a; DNMT; DNMT3A2; M HsaIIIA; M.HsaIIIA; MCMT; Methyl CpG binding domain protein 3a. Research Area Tumour Cell biology Signal transduction Apoptosis Epigenetics Immunogen Species Mouse Clonality Monoclonal Clone NO. G8L1 React Species Human, Applications WB=1:500-4000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 100kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen Recombinant human Dnmt3a. Lsotype IgG1,k Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016]
Function:
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
Subunit:
Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with UBC9, PIAS1 and PIAS2. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD domain. Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Interacts with MPHOSPH8. Interacts with histone H3 that is not methylated at 'Lys-4' (H3K4).
Subcellular Location:
Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin.
Tissue Specificity:
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
Post-translational modifications:
Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
Similarity:
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.
SWISS:
Q9Y6K1
Gene ID:
1788
Database links:Entrez Gene: 1788 Human
SwissProt: Q9Y6K1 Human
Product Picture
Scan Wechat Qrcode
Scan Whatsapp Qrcode