Product Name	MBD2
Chinese Name	去甲基化酶MBD2单克隆抗体
Alias	Demethylase; DMTase; MBD 2; MBD2_HUMAN; MBD2a; Methyl CpG binding domain protein 2; Methyl CpG binding protein MBD2; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY CO 41.
Research Area	Cell biology  Epigenetics
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	G7R2
React Species	Human,
Applications	WB=1:500-4000 IHC-P=1:25 IHC-F=1:25 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	43kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human MBD2 between 10-228 amino acids.
Lsotype	IgG1,k
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011] Function: Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters. Subcellular Location: Nucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase. Tissue Specificity: Highly expressed in brain, heart, kidney, stomach, testis and placenta. Similarity: Contains 1 MBD (methyl-CpG-binding) domain. SWISS: Q9UBB5 Gene ID: 8932 Database links: Entrez Gene: 8932 Human SwissProt: Q9UBB5 Human
Product Picture	Sample: Lane 1: K562 cell lysates Lane 2: MCF-7 cell lysates Lane 3: Jurkat cell lysates Primary: Anti-MBD2 (SLM-51671M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 50/34 kD Paraformaldehyde-fixed, paraffin embedded (human colon tissue sections); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MBD2) Monoclonal Antibody, Unconjugated (SLM-51671M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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