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Product Name GNMT Chinese Name 甘氨酸-N-甲基转移酶单克隆抗体 Alias EC 2.1.1.20; Epididymis secretory sperm binding protein Li 182mP; Glycine N methyltransferase; Glycine N-methyltransferase; GNMT_HUMAN; HEL S 182mP; OTTHUMP00000016412. Research Area Signal transduction The new supersedes the old Immunogen Species Mouse Clonality Monoclonal Clone NO. K8T2 React Species Human, Applications WB=1:500-4000 Flow-Cyt=1:25
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 32kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen Recombinant human GNMT between 1-295 amino acids. Lsotype IgG2a,k Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail GNMT catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). It plays a possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.
Function:
Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.
Subunit:
Homotetramer.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Abundant in liver.
DISEASE:
Defects in GNMT are the cause of glycine N-methyltransferase deficiency (GNMT deficiency) [MIM:606664]; also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases.
Similarity:
Belongs to the pyridoxine kinase family.
SWISS:
Q14749
Gene ID:
27232
Database links:Entrez Gene: 27232 Human
SwissProt: Q14749 Human
Product Picture Sample:
Lane 1: Human liver tissue lysates
Primary: Anti-GNMT (SLM-51666M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 34 kD
Blank control:U-2 OS.
Primary Antibody (green line): Mouse Anti-GNMT antibody (SLM-51666M)
Dilution: 1:25;
Isotype Control Antibody (blue line): Mouse IgG Secondary Antibody : Goat anti-mouse IgG-AF488
Dilution: 1:400 Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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