Product Name	GNMT
Chinese Name	甘氨酸-N-甲基转移酶单克隆抗体
Alias	EC 2.1.1.20; Epididymis secretory sperm binding protein Li 182mP; Glycine N methyltransferase; Glycine N-methyltransferase; GNMT_HUMAN; HEL S 182mP; OTTHUMP00000016412.
Research Area	Signal transduction  The new supersedes the old
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	K8T2
React Species	Human,
Applications	WB=1:500-4000 Flow-Cyt=1:25  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	32kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human GNMT between 1-295 amino acids.
Lsotype	IgG2a,k
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	GNMT catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). It plays a possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Function: Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Subunit: Homotetramer. Subcellular Location: Cytoplasm. Tissue Specificity: Abundant in liver. DISEASE: Defects in GNMT are the cause of glycine N-methyltransferase deficiency (GNMT deficiency) [MIM:606664]; also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases. Similarity: Belongs to the pyridoxine kinase family. SWISS: Q14749 Gene ID: 27232 Database links: Entrez Gene: 27232 Human SwissProt: Q14749 Human
Product Picture	Sample: Lane 1: Human liver tissue lysates Primary: Anti-GNMT (SLM-51666M) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 34 kD Blank control:U-2 OS. Primary Antibody (green line): Mouse Anti-GNMT antibody (SLM-51666M) Dilution: 1:25; Isotype Control Antibody (blue line): Mouse IgG Secondary Antibody : Goat anti-mouse IgG-AF488 Dilution: 1:400 Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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