Product Name	EIF2B3
Chinese Name	eIF2Bγ单克隆抗体
Alias	EI2BG_HUMAN; EIF 2B; EI2BG; eIF 2B GDP GTP exchange factor subunit gamma; eIF-2B GDP-GTP exchange factor subunit gamma; eIF2B-gamma; EIF2Bgamma; Eukaryotic translation initiation factor 2B subunit 3 gamma; Eukaryotic translation initiation factor 2B, gamma; eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa; OTTHUMP00000010262; OTTHUMP00000010263; Translation initiation factor eIF-2B subunit gamma.
Research Area	Cell biology  Neurobiology  Epigenetics  Alzheimer's
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	H11F4
React Species	Human,
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:25 ICC=1:25 IF=1:25 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human EIF2B3: 151-400/452
Lsotype	IgG1,k
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] Function: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Subunit: Complex of five different subunits; alpha, beta, gamma, delta and epsilon. DISEASE: Defects in EIF2B3 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. Similarity: Belongs to the eIF-2B gamma/epsilon subunits family. SWISS: Q9NR50 Gene ID: 8891 Database links: Entrez Gene: 8891 Human SwissProt: Q9NR50 Human
Product Picture	Sample: Lane 1: HL-60 cell lysates Lane 2: MCF-7 cell lysates Lane 3: U-2OS cell lysates Lane 4: K562 cell lysates Primary: Anti-EIF2B3 (SLM-51656M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 56 kD U-2 OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (EI2BG) monoclonal Antibody, Unconjugated (SLM-51656M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.Dylight® 554 Phalloidin (red) was used to stain the cell Cytoplasmic actin. Blank control:U-2 OS. Primary Antibody (green line): Mouse Anti-EI2BG antibody (SLM-51656M) Dilution: 1:25; Isotype Control Antibody (blue line): Mouse IgG Secondary Antibody : Goat anti-mouse IgG-AF488 Dilution: 1:400 Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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