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Product Name KANK1 Chinese Name KANK1单克隆抗体 Alias ANKRD 15; Ankyrin repeat domain 15; Ankyrin repeat domain containing protein 15; Ankyrin repeat domain-containing protein 15; DKFZp451G231; KANK; KANK1_HUMAN; KIAA0172; Kidney ankyrin repeat containing protein; Kidney ankyrin repeat-containing protein; KN motif and ankyrin repeat domain-containing protein 1; MGC43128. Research Area Tumour Cell biology Signal transduction The new supersedes the old Immunogen Species Mouse Clonality Monoclonal Clone NO. Y6S1 React Species Human, Applications WB=1:500-2000 IHC-P=1:25 IHC-F=1:25 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 65kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen Recombinant human KANK1. Lsotype IgG1,k Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Product Picture Sample:
Lane 1: Jurkat cell lysates
Lane 2: K562 cell lysates
Primary: Anti-KANK1 (SLM-51646M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 150 kD
Paraformaldehyde-fixed, paraffin embedded (Human heart tissue sections); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KANK1) Monoclonal Antibody, Unconjugated (SLM-51646M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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