Product Name	GARS
Chinese Name	GARS单克隆抗体
Alias	AP 4 A synthetase; Charcot Marie Tooth neuropathy 2D; Charcot Marie Tooth neuropathy neuronal type D; CMT2D; Diadenosine tetraphosphate synthetase; DSMAV; EC 6.1.1.14; Glycine tRNA ligase; Glycyl tRNA synthetase; GlyRS; HMN5; SMAD1; GARS_HUMAN.
Research Area	Chromatin and nuclear signals  Neurobiology
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	A2S3
React Species	Human,
Applications	WB=1:500-2000 Flow-Cyt=1:25  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	83kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human GARS between 35-305 amino acids.
Lsotype	IgG1,k
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. [provided by RefSeq, Jul 2008] Function: GARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease. Subcellular Location: Cytoplasmic. SWISS: P41250 Gene ID: 2617 Database links: Entrez Gene: 2617 Human SwissProt: P41250 Human
Product Picture	Sample: Lane 1: Ramos cell lysates Lane 2: Jurkat cell lysates Lane 3: HT-1080 cell lysates Primary: Anti-GARS (SLM-51616M) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 83 kD Observed band size: 83 kD Blank control: Hela. Primary Antibody (green line): Mouse Anti-GARS antibody (SLM-51616M) Dilution: 1:25; Isotype Control Antibody (blue line): Mouse IgG Secondary Antibody : Goat anti-mouse IgG-AF488 Dilution: 1:400 Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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