Product Name	PPP2R1B
Chinese Name	蛋白质磷酸酶2调节亚基1B单克隆抗体
Alias	2AAB_HUMAN; beta isoform of regulatory subunit A, protein phosphatase 2; PP2A A beta; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta; PP2A, subunit A, PR65 beta isoform; PP2A, subunit A, R1 beta isoform; PPP2R1B; PR65B; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform; protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform; Protein phosphatase 2 regulatory subunit A alpha isoform; protein phosphatase 2, structural/regulatory subunit A, beta isoform; Serine threonine protein phosphatase 65 kDa; serine/threonine protein phosphatase 2A 65 kDa regulatory subunit A, beta isoform; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, beta isoform; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform.
Research Area	Cell biology  Signal transduction  Cyclin  Kinases and Phosphatases
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	H6T2
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 IHC-P=1:25 IHC-F=1:25 Flow-Cyt=1:25 ICC=1:25 IF=1:25 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	65kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human PPP2R1B.
Lsotype	IgG1,Κ
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Function: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Subunit: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with SGOL1. Interacts with RAF1. Similarity: Belongs to the phosphatase 2A regulatory subunit A family. Contains 15 HEAT repeats. SWISS: P30154 Gene ID: 5519 Database links: Entrez Gene: 5519 Human SwissProt: P30154 Human
Product Picture	Sample: Lane 1: Jurkat cell lysates Lane 2: Human brain tissue lysates Lane 3: Mouse brain tissue lysates Lane 4: Mouse lung tissue lysates Lane 5: Rat brain tissue lysates Primary: Anti-PPP2R1B (SLM-51604M) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 60 kD Paraformaldehyde-fixed, paraffin embedded (Human spleen sections ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP2R1B) Monoclonal Antibody, Unconjugated (SLM-51604M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PPP2R1B) monoclonal Antibody, Unconjugated (SLM-51604M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes,Alexa Fluor® 555 conjugated with Phalloidin(red) was used to stain the cell Cytoplasmic actin.DAPI (blue) was used to stain the cell nuclei. Blank control:Jurkat. Primary Antibody (green line): Mouse Anti-PPP2R1B antibody (SLM-51604M) Dilution: 1:25; Isotype Control Antibody (blue line): Mouse IgG Secondary Antibody : Goat anti-mouse IgG-AF488 Dilution: 1:400 Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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