Product Name	CBX8
Chinese Name	染色质Binding proteinCbx8单克隆抗体
Alias	Chromobox protein homolog 8; HPC3; Pc3; Polycomb 3 homolog; RC1; Rectachrome 1; CBX8_HUMAN.
Research Area	Chromatin and nuclear signals  Epigenetics
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	B2W3
React Species	Human,
Applications	WB=1:500-1000 Flow-Cyt=1:25  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	43kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CBX8
Lsotype	IgG1
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Cbx8, also known as Pc3, is a member of the Polycomb gene family and is required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. Cbx8 contains a chromodomain and is able to modify chromatin and histones. Function: Component of a Polycomb group (PcG) multiproteinPRC1-like complex, a complex class required to maintain thetranscriptionally repressive state of many genes, including Hoxgenes, throughout development. PcG PRC1 complex acts via chromatinremodeling and modification of histones; it mediatesmonoubiquitination of histone H2A 'Lys-119', rendering chromatinheritably changed in its expressibility. Subunit: Component of a PRC1-like complex. Interacts with RING1RNF2, PCGF1, PCGF2, PCGF3, BMI1, PCGF5 AND PCGF6. Interacts withMLLT3 and histone H3. Interacts with PHC2. Subcellular Location: Nucleus. Similarity: Contains 1 chromo domain. SWISS: Q9HC52 Gene ID: 57332 Database links: Entrez Gene: 57332 Human SwissProt: Q9HC52 Human
Product Picture	Sample: Lane 1: 293 cell lysates Lane 2: Hela cell lysates Lane 3: A549 cell lysates Primary: Anti-CBX8 (SLM-51593M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 78 kD Blank control: A549. Primary Antibody (green line): Mouse Anti-CBX8(C-term) antibody (SLM-51593M) Dilution: 1:25; Isotype Control Antibody (blue line): Mouse IgG Secondary Antibody : Goat anti-mouse IgG-AF488 Dilution: 1:400 Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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