Product Name	USP5
Chinese Name	Ubiquitin羧基末端水解酶5单克隆抗体
Alias	Deubiquitinating enzyme 5; Isopeptidase T; ISOT 1; ISOT; Ubiquitin carboxyl-terminal hydrolase 5; Ubiquitin isopeptidase T; Ubiquitin specific peptidase 5 (isopeptidase T); Ubiquitin specific peptidase 5; Ubiquitin specific processing protease 5; Ubiquitin specific protease 5 (isopeptidase T); Ubiquitin specific protease 5 (ubiquitin isopeptidase T); Ubiquitin thioesterase 5; Ubiquitin thiolesterase 5; Ubiquitin-specific-processing protease 5; UBP5_HUMAN.
Research Area	Cell biology  Epigenetics  Ubiquitin
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	H4Y13
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	96kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human USP5.
Lsotype	IgG1,κ
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Profound changes in patterns of gene expression can result from relatively small changes in the concentrations of sequence specific transcription factors. Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010] Function: Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. Subunit: Interacts with TRIML1. Post-translational modifications: The N-terminus is blocked. The UniProt Consortium Similarity: Belongs to the peptidase C19 family. Contains 2 UBA domains. Contains 1 UBP-type zinc finger. SWISS: P45974 Gene ID: 8078 Database links: Entrez Gene: 8078 Human Entrez Gene: 22225 Mouse Entrez Gene: 297593 Rat Omim: 601447 Human SwissProt: P45974 Human SwissProt: P56399 Mouse
Product Picture	Sample: Lane 1: U251 cell lysates Lane 2: PC-3 cell lysates Lane 3: Mouse brain tissue lysates Lane 4: Rat brain tissue lysates Lane 5: Rat liver tissue lysates Primary: Anti-USP5 (SLM-51588M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 96 kD Observed band size: 110 kD

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