Product Name	Prohibitin
Chinese Name	阻抑素/Tumour抑制因子单克隆抗体
Alias	Mitochondrial Marker; PHB; PHB1; Prohibitin - Mitochondrial Marker; PHB_HUMAN.
Research Area	Tumour  Cell biology  Chromatin and nuclear signals  Apoptosis  transcriptional regulatory factor  Cell type markers  Zinc finger protein
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	E4F12
React Species	Human, Mouse, Rat,
Applications	WB=1:500-1000 IHC-P=1:25-50 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	30kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human Prohibitin between 1-205 amino acids.
Lsotype	IgG2b,k
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Function: Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. Subunit: Interacts with PHB2. Interacts with STOML2. Subcellular Location: Mitochondrion inner membrane. Tissue Specificity: Widely expressed in different tissues. Similarity: Belongs to the prohibitin family. SWISS: P35232 Gene ID: 5245 Database links: Entrez Gene: 5245 Human SwissProt: P35232 Human
Product Picture	Sample: Lane 1: 293 cell lysates Lane 2: MCF-7 cell lysates Lane 3: HepG2 cell lysates Lane 4: NIH/3T3 cell lysates Lane 5: Rat Liver tissue lysates Primary: Anti-Prohibitin (SLM-51570M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD Paraformaldehyde-fixed, paraffin embedded (Human stomach section); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Prohibitin) Monoclonal Antibody, Unconjugated (SLM-51570M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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