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Product Name FUBP3 Chinese Name FUBP3单克隆抗体 Alias Far upstream element (FUSE) binding protein 3; Far upstream element binding protein 3; Far upstream element-binding protein 3; FBP3; FLJ25229; FUBP 3; FUBP3_HUMAN; FUSE binding protein 3; FUSE-binding protein 3; OTTHUMP00000022369. Research Area Tumour Cell biology Signal transduction transcriptional regulatory factor Cell differentiation Epigenetics Immunogen Species Mouse Clonality Monoclonal Clone NO. Q2C12 React Species Human, Applications WB=1:500-1000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 62kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen Recombinant human FUBP3 (His-tagged). Lsotype IgG1 Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Activation of FUSE, the far-upstream element, is required for the proper ex-pression of the mammalian gene c-Myc in undifferentiated cells. The binding of FBP (FUSE-binding protein or Far upstream element binding protein) to FUSE is necessary for c-Myc expression, indicating that FBP functions as a growth-dependent regulator of c-Myc expression. Isolated from proliferating HL60 cells, FBP, FBP2, and FBP3 comprise a family of single-stranded DNA-binding proteins that specifically bind to FUSE elements. The FBP transcription factors share a conserved central DNA-binding domain and show significant homology in their carboxyl-terminal activation domains. Expression of FBP is detected in undifferentiated cells and is substantially decreased following cellular differentiation.
Function:
May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.
Subunit:
Interacts with YAF2 through the region encompassing the first and second zinc fingers. Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the DBINO domain of INO80. Interacts with EED and EZH2; the interactions are indicative for an association with the PRC2/EED-EZH2 complex.
Subcellular Location:
Nucleus.
Tissue Specificity:
Detected in a number of cell lines.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Contains 4 KH domains.
SWISS:
Q96I24
Gene ID:
8939
Database links:Entrez Gene: 8939 Human
SwissProt: Q96I24 Human
Product Picture Sample:
Lane 1: Hela cell lysates
Lane 2: U251 cell lysates
Primary: Anti-FUBP3 (SLM-51567M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 68 kD
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (FUBP3) monoclonal Antibody, Unconjugated (SLM-51567M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes,Alexa Fluor® 555 conjugated with Phalloidin(red) was used to stain the cell Cytoplasmic actin.
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