Product Name	RPS6KB2
Chinese Name	核糖体S6蛋白激酶β2单克隆抗体
Alias	70 kDa ribosomal protein S6 kinase 2; p70 beta; p70 ribosomal S6 kinase beta; p70 S6 kinase beta; p70 S6KB; p70 S6Kbeta; p70(S6K) beta; p70-beta; p70-S6KB; p70S6Kb; Ribosomal protein S6 kinase 70kDa, polypeptide 2; Ribosomal protein S6 kinase beta 2; Ribosomal protein S6 kinase beta-2; RPS6KB2; S6 kinase related kinase; S6 kinase-related kinase; S6K beta 2; S6K beta; S6K-beta; S6K2; Serine/threonine protein kinase 14 beta; Serine/threonine-protein kinase 14 beta; SRK; STK14B; KS6B2_HUMAN.
Research Area	Tumour  Signal transduction  Apoptosis  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	G8F7
React Species	Human, Mouse,
Applications	WB=1:500-2000 IHC-P=1:200-500 IF=1:10-50 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	53kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human RPS6KB2 (His-tagged).
Lsotype	IgG1,K
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Function: Phosphorylates specifically ribosomal protein S6. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. SWISS: Q9UBS0 Gene ID: 6199 Database links: Entrez Gene: 6199 Human SwissProt: Q9UBS0 Human
Product Picture	Sample: Lane 1: Mouse NIH/3T3 cell lysates Primary: Anti-RPS6KB2 (SLM-51475M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 68 kD Sample: Lane 1: Jurkat cell lysates Primary: Anti-RPS6KB2 (SLM-51475M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 68 kD Paraformaldehyde-fixed, paraffin embedded ( human hepatocarcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RPS6KB2) Monoclonal Antibody, Unconjugated (SLM-51475M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Blank control:Jurkat. Primary Antibody (green line): Mouse Anti-RPS6KB2 antibody (SLM-51475M) Dilution: 1:50; Secondary Antibody : Goat anti-mouse IgG-FITC Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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