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Product Name ALDH6A1 Chinese Name ALDH6A1单克隆抗体 Alias Aldehyde dehydrogenase 6 family member A1; Aldehyde dehydrogenase family 6 member A1; Aldehyde dehydrogenase family 6, subfamily A1; Malonate semialdehyde dehydrogenase; Malonate-semialdehyde dehydrogenase [acylating]; Methylmalonate semialdehyde dehydrogenase [acylating] mitochondrial; Methylmalonate-semialdehyde dehydrogenase [acylating]; MGC37325; MGC40271; Mitochondrial acylating methylmalonate semialdehyde dehydrogenase; mitochondrial; MMSA_HUMAN; MMSADHA; MMSDH; Research Area Signal transduction Growth factors and hormones The new supersedes the old Immunogen Species Mouse Clonality Monoclonal Clone NO. AL06 React Species Human, Applications WB=1:500-1000 IHC-P=1:25 ICC=1:25 IF=1:25 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 59kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen Recombinant human ALDH6A1. Lsotype IgG1,IgK Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the aldehyde dehydrogenase protein family. The encoded protein is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This protein catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Function:
Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
Subunit:
Homotetramer.
Subcellular Location:
Mitochondrion.
Similarity:
Belongs to the aldehyde dehydrogenase family.
SWISS:
Q02252
Gene ID:
4329
Database links:
Entrez Gene: 4329 Human
SwissProt: Q02252 Human
Product Picture Sample:
Lane 1: MCF-7 cell lysates
Lane 2: T47D cell lysates
Primary: Anti-ALDH6A1 (SLM-51469M) at 1:1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 56 kD
Paraformaldehyde-fixed, paraffin embedded ( human LIVER section); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALDH6A1) Monoclonal Antibody, Unconjugated (SLM-51469M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded ( human colon section); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALDH6A1) Monoclonal Antibody, Unconjugated (SLM-51469M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ALDH6A1) monoclonal Antibody, Unconjugated (SLM-51469M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Alexa Fluor® 555 conjugated with Phalloidin(red) was used to stain the cell Cytoplasmic actin .
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