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Product Name NUP54 Chinese Name NUP54单克隆抗体 Alias 54 kDa nucleoporin; MGC13407; Nucleoporin p54; NUP54_HUMAN. Research Area Tumour Cell biology transcriptional regulatory factor Zinc finger protein Immunogen Species Mouse Clonality Monoclonal Clone NO. N4S1 React Species Human, Applications IHC-P=1:400-800 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 55kDa Cellular localization The nucleus cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen Recombinant human NUP54. Lsotype IgG1,Igκ Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail NUP54 contains FG repeats and it belongs to the NUP54 family. It is a component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane.
Function:
Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane
Subunit:
Component of the p62 complex, a complex composed of NUP62, NUP54, and the isoform p58 and isoform p45 of NUPL1
Subcellular Location:
Nucleus, nuclear pore complex (By similarity). Nucleus membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side (By similarity). Note=Biased towards cytoplasmic side. Central region of the nuclear pore complex, within the transporter
Post-translational modifications:
O-glycosylated
Similarity:
Belongs to the NUP54 family.
SWISS:
Q7Z3B4
Gene ID:
53371
Database links:Entrez Gene: 53371 Human
SwissProt: Q7Z3B4 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUP54 ) Monoclonal Antibody, Unconjugated (SLM-51465M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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