Product Name	SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized
Chinese Name	SARS冠状病毒2核衣壳蛋白单克隆抗体（新冠抗原检测原料，可识别奥密克戎）
Alias	SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein; Ormicron mutant; Ormicron Nucleocapsid Protein; Ormicron N Protein.
Research Area	Bacteria and viruses
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	9D2
Applications	WB=1:500-2000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	46kDa
Form	Liquid
immunogen	Recombinant SARS-CoV-2 Nucleocapsid Protein: 1-419/419
Lsotype	IgG1
Purification	Affinity purified by Protein A
Buffer Solution	0.01M PBS(pH7.4)
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. SWISS: P0DTC9 Gene ID: 43740575
Product Picture	Sample: Lane 1: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng Lane 2: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng Lane 3: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng Lane 4: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng Lane 5: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng Lane 6: Recombinant SARS-CoV-2 N Protein(WT) at 10ng Primary: Mouse Anti- SARS-CoV-2 N protein (SLM-41481M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size:45.3 kD Observed band size:50 kD Sample: Lane 1: SARS-CoV-2 N protein(WT) at 10ng Lane 2: SARS-CoV-2 N protein(Q9H, P67S, P80R, P151L, S183Y) at 2ng Lane 3: SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y,H145Y, R203K, G204R, T205I, S235F) at 2ng Lane 4: SARS-CoV-2 N protein(Del204 & Del215) at 2ng Lane 5: SARS-CoV-2 N protein(R203M & D377Y) at 2ng Primary: Anti- SARS-CoV-2 N protein at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD Sample: Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) (SL41408P) Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) (SL41451P) Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) (SL41452P) Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) (SL41491P) Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) (SL41492P) Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) (SL41494P) Primary: Anti-SARS-CoV-2(2019-nCoV)Nucleocapsid, WT & Ormicron Recognized (SLM-41481M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 50 kDa

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