Mouse Anti-AMPK b1 antibody
5 AMP activated protein kinase subunit beta 1; AMPK; AMPK beta 1 chain; AMPKb; HAMPKb; PRKAB1; 5'-AMP-activated protein kinase subunit beta-1; AMP-activated protein kinase beta subunit; protein kinase, AMP-activated, noncatalytic, beta-1; AMPK beta -1 cha
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Product Name AMPK b1 Chinese Name 腺苷单磷酸活化蛋白激酶β1单克隆抗体 Alias 5 AMP activated protein kinase subunit beta 1; AMPK; AMPK beta 1 chain; AMPKb; HAMPKb; PRKAB1; 5'-AMP-activated protein kinase subunit beta-1; AMP-activated protein kinase beta subunit; protein kinase, AMP-activated, noncatalytic, beta-1; AMPK beta -1 chain; 5'-AMP-activated protein kinase beta-1 subunit; AMPKb; AMPK subunit beta-1; AAKB1_HUMAN; AMPK b1; AMPK-b1. literatures Research Area Cell biology immunology Signal transduction Immunogen Species Mouse Clonality Monoclonal Clone NO. 3G2 React Species Human, Mouse, Rat, Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 30kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen Recombinant human AMPK b1 Protein: 118-226/272 Lsotype IgG1 Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008].
Function:
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Subunit:
AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.
Tissue Specificity:
Highly expressed in kidney, heart, white adipose tissue, lung and spleen.
Post-translational modifications:
Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
Similarity:
Belongs to the 5'-AMP-activated protein kinase beta subunit family.
SWISS:
Q9Y478
Gene ID:
5564
Database links:Entrez Gene: 5564 Human
Entrez Gene: 19079 Mouse
Omim: 602740 Human
SwissProt: Q9Y478 Human
SwissProt: Q9R078 Mouse
Unigene: 6061 Human
Unigene: 726001 Human
Unigene: 458152 Mouse
Unigene: 3619 Rat
Product Picture 
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
U87MG(Human) Cell Lysate at 30 ug
U251(Human) Cell Lysate at 30 ug
Primary: Anti- AMPK b1 (SLM-33426M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 38 kD
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- AMPK b1 (SLM-33426M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 38 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AMPK b1) Monoclonal Antibody, Unconjugated (SLM-33426M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
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