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Product Name ATG5/APG5L Chinese Name 自噬蛋白5单克隆抗体 Alias ATG5; APG 5; APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; APG5L; Apoptosis specific protein; Apoptosis-specific protein; ASP; ATG 5; ATG5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5; Homolog of S Cerevisiae autophagy 5; ATG5_HUMAN. literatures Specific References (1) | SLM-33385M has been referenced in 1 publications.[IF=7.963] Jianzhao Liao. et al. Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis. Sci Total Environ. 2022 Feb;809:152188 WB ; Mouse.Research Area Cell biology Apoptosis Autophagy Immunogen Species Mouse Clonality Monoclonal Clone NO. 5D7 React Species Human, Rat, (predicted: Mouse, ) Applications WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 32kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen Recombinant human ATG5 Protein: full length Lsotype IgG Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis-specific protein or APS), function as substrates for the autophagy protein Apg12. These proteins are covalently bonded together to form Apg12/APG5 conjugates, which are required for the progression of autophagy.
Function:
Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity).
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Subunit:
The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5-ATG12 conjugate.
Subcellular Location:
Cytoplasm. Note=Colocalizes with nonmuscle actin.
Tissue Specificity:
Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
Post-translational modifications:
Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
Similarity:
Belongs to the ATG5 family.
SWISS:
Q9H1Y0
Gene ID:
9474
Database links:Entrez Gene: 9474 Human
Entrez Gene: 11793 Mouse
Omim: 604261 Human
SwissProt: Q9H1Y0 Human
SwissProt: Q99J83 Mouse
Unigene: 486063 Human
Unigene: 22264 Mouse
Unigene: 98385 Rat
Product Picture Sample:
Lane 1: Human Hela cell lysates
Lane 2: Human Panc-1 cell lysates
Lane 3: Human U87MG cell lysates
Lane 4: Human MCF-7 cell lysates
Lane 5: Human K562 cell lysates
Lane 6: Human SH-SY5Y cell lysates
Primary: Anti- ATG5/APG5L (SLM-33385M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 45 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5/APG5L) Monoclonal Antibody, Unconjugated (SLM-33385M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5) Monoclonal Antibody, Unconjugated (ascites of SLM-33385M 5D7) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ATG5/APG5L) polyclonal Antibody, Unconjugated (SLM-33385M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ATG5/APG5L) monoclonal Antibody, Unconjugated (SLM-33385M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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