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Product Name MLKL Chinese Name 混合系列蛋白激酶样结构域单克隆抗体 Alias hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain-like protein; mixed lineage kinase domain like pseudokinase; MLKL_HUMAN. literatures Specific References (2) | SLM-33339M has been referenced in 2 publications.[IF=6.792] Lu Peng. et al. Polychlorinated biphenyl quinone regulates MLKL phosphorylation that stimulates exosome biogenesis and secretion via a short negative feedback loop. Environ Pollut. 2021 Apr;274:115606 WB ; Human.[IF=3.274] Yang B et al. Polychlorinated Biphenyl Quinone Promotes Macrophage-Derived Foam Cell Formation. Chem Res Toxicol. 2019 Nov 13. WB ; Mouse.Research Area Cell biology Kinases and Phosphatases Immunogen Species Mouse Clonality Monoclonal Clone NO. 10H7 React Species (predicted: Human, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 54kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MLKL Lsotype IgG1 Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015].
Function:
Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.
Subunit:
Homotrimer; forms homotrimers on necroptosis induction. Interacts with RIPK3; the interaction is direct. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5.
Subcellular Location:
Cytoplasm. Cell membrane. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.
Post-translational modifications:
Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.
Similarity:
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
SWISS:
Q8NB16
Gene ID:
197259
Database links:Entrez Gene: 197259 Human
Entrez Gene: 74568 Mouse
Omim: 615153 Human
SwissProt: Q8NB16 Human
SwissProt: Q9D2Y4 Mouse
Unigene: 119878 Human
Unigene: 207971 Mouse
Unigene: 105677 Rat
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