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Mouse Anti-Firefly Luciferase antibody
Mouse Anti-Firefly Luciferase antibody
ec 1.13.12.7; Firefly; Firefly Luciferase Luciferase; Luciferin 4 monooxygenase.
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Details

Product Name Firefly Luciferase
Chinese Name 荧光素酶单克隆抗体
Alias ec 1.13.12.7; Firefly; Firefly Luciferase Luciferase; Luciferin 4 monooxygenase.  
literatures
Specific References  (1)     |     SLM-33318M has been referenced in 1 publications.
[IF=6.58] Jiang Chen. et al. Inhibition of SARS-CoV-2 pseudovirus invasion by ACE2 protecting and Spike neutralizing peptides: An alternative approach to COVID19 prevention and therapy. Int J Biol Sci. 2021; 17(11): 2957–2969  IF,IHC ;  Human.  
Product Type Tag anti 
Research Area Insect  
Immunogen Species Mouse
Clonality Monoclonal
Clone NO. 11D6
React Species Recombinant protein
Applications WB=1:500-5000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 61kDa
Cellular localization cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen Luciferase comes from the North American firefly (Firefly) 
Lsotype IgG1
Purification affinity purified by Protein G
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Function:
Produces green light with a wavelength of 562 nm.

Subcellular Location:
Peroxisome.

Similarity:
Belongs to the ATP-dependent AMP-binding enzyme family.

SWISS:
P08659

Gene ID:
N/A

Database links:

SwissProt: P08659 Firefly (Photinus pyralis)



Product Picture
Sample: Fruit Fly Lysate at 40 ug
Primary: Anti-Firefly Luciferase (SLM-33318M) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD

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