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Product Name Epsilon Tubulin Chinese Name ε-微管蛋白单克隆抗体 Alias dJ142L7.2; Epsilon-tubulin; FLJ22589; FLJ44203; OTTHUMP00000040425; TBE_HUMAN; TUBE 1; TUBE; TUBE1; Tubulin epsilon 1; Tubulin epsilon; Tubulin epsilon chain. Research Area Cell biology Neurobiology Cytoskeleton Immunogen Species Mouse Clonality Monoclonal Clone NO. 4C3 React Species Human, Mouse, (predicted: Rat, ) Applications WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 53kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Epsilon Tubulin Lsotype IgG Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the tubulin superfamily. This protein localizes to the centriolar sub-distal appendages that are associated with the older of the two centrioles after centrosome duplication. This protein plays a central role in organization of the microtubules during centriole duplication. A pseudogene of this gene is found on chromosome 5.[provided by RefSeq, Jan 2009]
Subcellular Location:
Cytoplasm; cytoskeleton; centrosome. Associated with pericentriolar material.
Similarity:
Belongs to the tubulin family.
SWISS:
Q9UJT0
Gene ID:
51175
Database links:Entrez Gene: 51175 Human
Omim: 607345 Human
SwissProt: Q9UJT0 Human
Unigene: 34851 Human
Product Picture Sample:
K562(Human) Cell Lysate at 30 ug
HL60(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- Epsilon Tubulin (SLM-33314M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBE) Polyclonal Antibody, Unconjugated (SLM-33314M-4C3) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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