Mouse Anti-Transferrin (Serum Loading Control)antibody
Apotransferrin; TRF; eta 1 metal binding globulin; DKFZp781D0156; PRO1400; PRO1557; PRO2086; Serotransferrin precursor; Siderophilin; TF; TRFE_HUMAN; Beta-1 metal-binding globulin; Serotransferrin.
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Product Name Transferrin(Serum Loading Control) Chinese Name 转铁蛋白（内参）单克隆抗体 Alias Apotransferrin; TRF; eta 1 metal binding globulin; DKFZp781D0156; PRO1400; PRO1557; PRO2086; Serotransferrin precursor; Siderophilin; TF; TRFE_HUMAN; Beta-1 metal-binding globulin; Serotransferrin. Product Type Internal reference anti Research Area Cardiovascular Immunogen Species Mouse Clonality Monoclonal Clone NO. 12A7 React Species Human, Rat, Applications WB=1:500-1000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test （Paraffin sections need antigen repair）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 77kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen Full length native Transferrin protein purified from human plasma Lsotype IgG Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009].
Function:
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Subunit:
Monomer.
Subcellular Location:
Secreted.
Tissue Specificity:
Expressed by the liver and secreted in plasma.
DISEASE:
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
Similarity:
Belongs to the transferrin family.
Contains 2 transferrin-like domains.
SWISS:
P02787
Gene ID:
7018
Database links:Entrez Gene: 7018 Human
Entrez Gene: 22041 Mouse
Omim: 190000 Human
SwissProt: P02787 Human
SwissProt: Q921I1 Mouse
Unigene: 518267 Human
Unigene: 37214 Mouse
Unigene: 91296 Rat
Product Picture 
Sample:
Lane 1: Serum (Rat) at 40 ug
Lane 2: Plasma (Rat) at 40 ug
Primary: Anti-Transferrin (SLM-33243M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 77 kD
Observed band size: 75 kD
Sample:
Lane 1: Human serum
Primary: Anti-Transferrin (SLM-33243M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa
Sample:
Lane 1: Serum (Human) at 20 ug
Primary: Anti-Transferrin (SLM-33243M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 77 kD
Observed band size: 77 kD
Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Transferrin) Monoclonal Antibody, Unconjugated (SLM-33243M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Transferrin) Monoclonal Antibody, Unconjugated (SLM-33243M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Blank control（black line）:HepG2.
Primary Antibody (green line): Mosue Anti-Transferrin(Serum Loading Control) antibody (SLM-33243M)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-mouse IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Mouse IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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