Product Name	[KO validated anti] STAT3
Chinese Name	Signal transduction和转录激活因子3单克隆抗体
Alias	1110034C02Rik; Acute Phase Response Factor; APRF; AW109958; DNA binding protein APRF; FLJ20882; HIES; MGC128731; MGC16063; MGC93551; Signal transducer and activator of transcription 3 (acute-phase response factor); Signal transducer and activator of transcription 3; Signal Transductor and Activator of Transcription 3; STAT 3; Transcription factor; STAT3_HUMAN.
literatures	Specific References  (7)     |     SLM-33223M has been referenced in 7 publications. [IF=5.595] Zhao W et al. Long noncoding RNA NSCLCAT1 increases non-small cell lung cancer cell invasion and migration through the Hippo signaling pathway by interacting with CDH1.(2018)FASEB J. Aug 27  IHC ;  Human.   PubMed:30148675 [IF=5.546] Feng‐Feng Lv. et al. microRNA‐495 alleviates endothelial cell inflammatory injury in coronary heart disease via blockade of the NF‐κB pathway by targeting RALB. 2020 Aug 18  IHC ;  Mouse.   PubMed:32810308 [IF=3.923] Zhao Z et al.Protective role of microRNA-374 against myocardial ischemia-reperfusion injury in mice following thoracic epidural anesthesia by downregulating dystrobrevin alpha-mediated Notch1 axis.(2018)J Cell Physiol. Nov 22.  IHC ;  Mouse.   PubMed:30565678 [IF=3.287] Keliang Song. et al. Knocking down Siglec-15 in osteosarcoma cells inhibits proliferation while promoting apoptosis and pyroptosis by targeting the Siglec-15/STAT3/Bcl-2 pathway. ADV MED SCI-POLAND. Adv Med Sci-Poland. 2022 Sep;67:187  WB ;  Human.   PubMed:35398779 [IF=2.923] Taojie Zhang. et al. MEL regulates miR-21 and let-7b through the STAT3 cascade in the follicular granulosa cells of Tibetan sheep. THERIOGENOLOGY. 2023 Apr;:  IF ;  Sheep.   PubMed:37120893 [IF=2.559] Lin JC et al. MicroRNA-874 targeting SUFU involves in osteoblast proliferation and differentiation in osteoporosis rats through the Hedgehog signaling pathway.(2018)Biochem. Biophys. Res. Commun.Nov 17;506(1):194-203.  IHC ;  Rat.   PubMed:30342851 [IF=2.524] Wu Y et al. Silencing of RAD51AP1 suppresses epithelial–mesenchymal transition and metastasis in non‐small cell lung cancer. Thorac Cancer. 2019 Jul 17.  IHC-P ;  Human.   PubMed:31317661
Research Area	Tumour  Cell biology  Signal transduction  Apoptosis  transcriptional regulatory factor
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	5H4
React Species	Human, Mouse,  (predicted: Rat, )
Applications	WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	88kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human STAT3 Protein:  9-265/770
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. Gene Symbol: APRF; FLJ20882; HIES; MGC16063. Subunit: Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1 and TMF1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts (via SH2 domain) with NLK. Interacts with ARL2BP; the interaction is enhanced by LIF and JAK1 expression (By similarity). Interacts with KPNA4 and KPNA5; KPNA4 may be the primary mediator of nuclear import (By similarity). Interacts with CAV2; the interaction is increased on insulin-induced tyrosine phosphorylation of CAV2 and leads to STAT3 activation (By similarity). Interacts with ARL2BP; interaction is enhanced with ARL2. Interacts with NEK6 (By similarity). Binds to CDK9 when activated and nuclear. Interacts with BMX. Interacts with ZIPK/DAPK3. Interacts with PIAS3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. In prostate cancer cells, interacts with STAT3 and promotes DNA binding activity of STAT3. Interacts with STMN3, antagonizing its microtubule-destabilizing activity. Subcellular Location: Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1. Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Similarity: Belongs to the transcription factor STAT family. Contains 1 SH2 domain. SWISS: P40763 Gene ID: 6774 Database links: Entrez Gene: 6774 Human Entrez Gene: 20848 Mouse Entrez Gene: 25125 Rat Omim: 102582 Human SwissProt: P40763 Human SwissProt: P42227 Mouse SwissProt: P52631 Rat Stat3在Cell differentiation、增殖、凋亡等方面有着重要生理作用。有学者认为：STAT-3对Tumour有一定的抑制作用，由于其与Tumour的关系较为密切，越来越受到人们的重视。
Product Picture	Sample: Lane 1: Mouse NIH/3T3 cell lysates Lane 2: Mouse Raw264.7 cell lysates Lane 3: Human HepG2 cell lysates Lane 4: Human HeLa cell lysates Lane 5: Human SW480 cell lysates Lane 6: Human THP-1 cell lysates Primary: Anti-STAT3 (SLM-33223M) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 88 kDa Observed band size: 92 kDa Sample: Heart (Mouse) Lysate at 40 ug Placenta (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Primary: Anti- STAT3 (SLM-33223M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 88 kD Sample: Lane 1: STAT3 knockout (KO) A549 Cell Lysate Lane 2: Human A549 Cell (Control) Lysate Primary: Anti-STAT3 (SLM-33223M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 88 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STAT3) Monoclonal Antibody, Unconjugated (SLM-33223M-5H4) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STAT3) Monoclonal Antibody, Unconjugated (SLM-33223M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STAT3) Monoclonal Antibody, Unconjugated (SLM-33223M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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