Product Name	TNF alpha
Chinese Name	Tumour坏死因子-α单克隆抗体
Alias	Tumor necrosis factor-α; TNFalpha; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a; TNF-alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor Alpha.
literatures	Specific References  (19)     |     SLM-33207M has been referenced in 19 publications. [IF=32.086] Yingying Jin. et al. A Novel “Inside-Out” Intraocular Nanomedicine Delivery Mode for Nanomaterials Biological Effects Enhanced Choroidal Neovascularization Occlusion and Microenvironment Regulation. ADV MATER. 2022 Dec;:2209690  IF ;  Mouse.   PubMed:36527723 [IF=14.224] Biqi Han. et al. Effects of thiacloprid exposure on microbiota–gut–liver axis: Multiomics mechanistic analysis in Japanese quails. J HAZARD MATER. 2023 Jan;442:130082  WB ;  Quail.   PubMed:36209609 [IF=12.153] Hong Jihye. et al. Senescent cancer cell-derived nanovesicle as a personalized therapeutic cancer vaccine. EXP MOL MED. 2023 Mar;:1-14  WB ;  Mouse.   PubMed:36854774 [IF=7.182] Tingting Guo. et al. Lepidium meyenii Walpers polysaccharide and its cationic derivative re-educate tumor-associated macrophages for synergistic tumor immunotherapy. Carbohyd Polym. 2020 Dec;250:116904  IF ;  Mouse.   PubMed:33049880 [IF=6.656] Hongya Guo. et al. Gua Lou Er Chen Decoction Attenuates Atherosclerosis by Reducing Proteoglycans Accumulation and Inflammation. PHYTOMEDICINE. 2023 Apr;:154811  WB ;  Mouse.   PubMed:37094421 [IF=6.208] Albert chun-shuo Huang. et al. NF-κB Decoy ODN-Loaded Poly(Lactic-co-glycolic Acid) Nanospheres Inhibit Alveolar Ridge Resorption. INT J MOL SCI. 2023 Jan;24(4):3699  IHC ;  Rat.   PubMed:36835111 [IF=5.988] Sisi Lei. et al. Chaihu Shugan powder alleviates liver inflammation and hepatic steatosis in NAFLD mice: A network pharmacology study and in vivo experimental validation.. FRONT PHARMACOL. 2022 Sep;13:967623-967623  WB ;  Mouse.   PubMed:36172180 [IF=5.195] Yanting You. et al. Jiawei Yanghe Decoction suppresses breast cancer by regulating immune responses via JAK2/STAT3 signaling pathway. J ETHNOPHARMACOL. 2023 Mar;:116358  IHC,WB ;  Mouse.   PubMed:36933872 [IF=5.168] Huimin Ouyang. et al. Fumonisin B1 promotes germ cells apoptosis associated with oxidative stress-related Nrf2 signaling in mice testes. CHEM-BIOL INTERACT. 2022 Aug;363:110009  WB ;  Mouse.   PubMed:35697133 [IF=4.757] Rachana Pandit. et al. Canine Coronavirus Infection Modulates the Biogenesis and Composition of Cell-Derived Extracellular Vesicles. BIOMEDICINES. 2023 Mar;11(3):976  Dot Blot ;  Cat.   PubMed:36979955 [IF=4.294] Yang Hu. et al. Astragalin attenuates AlCl3/D-galactose-induced aging-like disorders by inhibiting oxidative stress and neuroinflammation. NEUROTOXICOLOGY. 2022 Jul;91:60  IHC ;  Mouse.   PubMed:35537655 [IF=4.294] Sheng-Rui Fan. et al. Edaravone attenuates cadmium-induced toxicity by inhibiting oxidative stress and inflammation in ICR mice. Neurotoxicology. 2021 Sep;86:1  IHC ;  Mouse.   PubMed:34174317 [IF=4.133] Huang G et al. Potential role of full-length and nonfull-length progranulin in affecting aortic valve calcification. J Mol Cell Cardiol . 2020 Apr;141:93-104.  WB ;  human.   PubMed:32247641 [IF=4.014] M. Zhang. et al. Osteocalcin reduces fat accumulation and inflammatory reaction by inhibiting ROS-JNK signal pathway in chicken embryonic hepatocytes. POULTRY SCIENCE. 2022 Jun;:102026  WB ;   PubMed:10.1016/j.psj.2022.102026 [IF=3.352] Yusong Miao. et al. Methylsulfonylmethane ameliorates inflammation via NF-κB and ERK/JNK-MAPK signaling pathway in chicken trachea and HD11 cells during Mycoplasma gallisepticum infection. Poultry Sci. 2022 Jan;:101706  WB ;  Chicken.   PubMed:35121233 [IF=3.105] Huan-Tong Wu. et al. Edaravone attenuates H2O2 or glutamate-induced toxicity in hippocampal neurons and improves AlCl3/D-galactose induced cognitive impairment in mice. Neurotoxicology. 2021 Jul;85:68  IHC ;  Rat.   PubMed:34004234 [IF=3.046] Dan Tang. et al. Treadmill training improves respiratory function in rats after spinal cord injury by inhibiting the HMGB1/TLR-4/NF-κB signaling pathway. NEUROSCI LETT. 2022 May;:136686  WB ;  Rat.   PubMed:35595191 [IF=2.992] Xinjia Han. et al. Nicotine Alleviates Cortical Neuronal Injury by Suppressing Neuroinflammation and Upregulating Neuronal PI3K-AKT Signaling in an Eclampsia-Like Seizure Model. Neurotox Res. 2020 Oct;38(3):665-681  IF ;  Mouse.   PubMed:32767216 [IF=2.742] Liu, Yan. et al. Luteolin alleviates inorganic mercury-induced liver injury in quails by resisting oxidative stress and promoting mercury ion excretion. MOL BIOL REP. 2022 Nov;:1-10  WB ;  Quail.   PubMed:36336778
Research Area	Cytoskeleton  Extracellular matrix
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	3A4
React Species	Rat,  (predicted: Mouse, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, Monkey, )
Applications	WB=1:1000-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:200-500 ICC=1:100-500 IF=1:200-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17/26kDa
Cellular localization	The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human TNF alpha 77-233aa
Lsotype	IgG1
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020] Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. Subunit: Homotrimer. Interacts with SPPL2B.. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor, membrane form: Membrane; Single-pass type II membrane protein. Tumor necrosis factor, soluble form: Secreted. C-domain 1: Secreted. C-domain 2: Secreted. Post-translational modifications: The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. DISEASE: Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Similarity: Belongs to the tumor necrosis factor family. SWISS: P01375 Gene ID: 7124 Database links: Entrez Gene: 7124 Human Entrez Gene: 21926 Mouse Omim: 191160 Human SwissProt: P01375 Human SwissProt: P06804 Mouse Unigene: 241570 Human Unigene: 1293 Mouse
Product Picture	Sample: plasma (Rat) at 40 ug serum (Rat) at 40 ug Primary: Anti-TNF-alpha (SLM-33207M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 17/26 kD Observed band size: 26 kD

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