Product Name	CBARA1/MICU1
Chinese Name	Mitochondrion钙吸收蛋白单克隆抗体
Alias	Mitochondrial Calcium Uptake 1; Calcium-Binding Atopy-Related Autoantigen 1; Atopy-Related Autoantigen CALC; Ara CALC; CBARA1; CALC; Calcium Binding Atopy-Related Autoantigen 1; Calcium Uptake Protein 1, Mitochondrial; Allergen Hom S 4; EFHA3; MPXPS; MICU1_HUMAN;
Research Area	Cell biology  Signal transduction  The new supersedes the old  Mitochondrion
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	1H8
React Species	Human,  (predicted: Mouse, Rat, )
Applications	WB=1:500-1000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	54kDa
Cellular localization	cytoplasmic Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human CBARA1 Protein (1-250/476)
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes an essential regulator of mitochondrial Ca2+ uptake under basal conditions. The encoded protein interacts with the mitochondrial calcium uniporter, a mitochondrial inner membrane Ca2+ channel, and is essential in preventing mitochondrial Ca2+ overload, which can cause excessive production of reactive oxygen species and cell stress. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2013] Function: Key regulator of mitochondrial calcium uniporter (MCU) that senses calcium level via its EF-hand domains (PubMed:20693986, PubMed:23101630, PubMed:23747253, PubMed:24313810, PubMed:24332854, PubMed:24503055, PubMed:24560927, PubMed:26341627, PubMed:26903221, PubMed:27099988). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium. MICU1 acts both as an activator or inhibitor of mitochondrial calcium uptake (PubMed:26903221). Acts as a gatekeeper of MCU at low concentration of calcium, preventing channel opening (PubMed:26903221). Enhances MCU opening at high calcium concentration, allowing a rapid response of mitochondria to calcium signals generated in the cytoplasm (PubMed:24560927, PubMed:26903221). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed:22904319). Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG (PubMed:16002733). Subunit: Homohexamer; in absence of calcium (PubMed:24514027). Forms a homohexamer in absence of calcium and rearranges into a heterodimer in presence of calcium (PubMed:26489515, PubMed:24514027). Heterodimer; disulfide-linked; heterodimerizes with MICU2 (PubMed:24560927). The heterodimer formed with MICU2 associates with MCU at low calcium concentration and dissociates from MCU at high calcium level (PubMed:26387864). Component of the uniplex complex, composed of MCU, MCUB, MICU1, MICU2 and EMRE/SMDT1 (PubMed:24231807). Interacts (via polybasic region) with EMRE/SMDT1; the interaction is direct (PubMed:27099988). Interacts (via polybasic region) with MCU (via coiled coil domains); the interaction is direct and precedes formation of the heterodimer with MICU2 (PubMed:21685886, PubMed:23101630, PubMed:23178883, PubMed:24332854, PubMed:26387864). Interacts with SLC25A23 (PubMed:24430870). Interacts with CHCHD4/MIA40; which introduces the interchain disulfide bond with MICU2 (PubMed:26387864). Subcellular Location: Mitochondrion inner membrane; Single-pass membrane protein; Mitochondrion intermembrane space Tissue Specificity: Expressed in epithelial cell lines. Strongly expressed in epidermal keratinocytes and dermal endothelial cells. Similarity: Belongs to the MICU1 family. MICU1 subfamily. Contains 2 EF-hand domains. SWISS: Q9BPX6 Gene ID: 10367 Database links: Entrez Gene: 507899 Cow Entrez Gene: 479241 Dog Entrez Gene: 10367 Human Entrez Gene: 216001 Mouse Entrez Gene: 365567 Rat Omim: 605084 Human SwissProt: Q0IIL1 Cow SwissProt: Q9BPX6 Human SwissProt: Q8VCX5 Mouse SwissProt: Q6P6Q9 Rat Unigene: 524367 Human Unigene: 27199 Mouse Unigene: 214252 Rat
Product Picture	Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: U937 (Human) Cell Lysate at 30 ug Lane 3: U87MG (Human) Cell Lysate at 30 ug Lane 4: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti- CBARA1/MICU1 (SLM-33198M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 54/46 kD Observed band size: 54/46 kD

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