Product Name	HAO1
Chinese Name	葡萄糖氧化酶1单克隆抗体
Alias	Glycolate oxidase; GOX; GOX1; HAO1; HAOX1; HAOX1_HUMAN; Hydroxyacid oxidase 1; MGC142225; MGC142227; OTTHUMP00000030231; (S) 2 hydroxy acid oxidase.
Research Area	Tumour  Cardiovascular  Cell biology  Signal transduction
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	6G8
React Species	Mouse, Rat,
Applications	WB=1:500-2000 IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	41kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant mouse HAO1 Protein: full length
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	GOX is a 370 amino acid protein that is expressed in liver and pancreas. HAO1 is localized to peroxisomes and aids in organic acid metabolism via 2-hydroxyacid oxidase activity. 2-hydroxyacid oxidases, such as HAO1, are enzymes that require a flavin cofactor to oxidize 2-hydroxyacids to 2-ketoacids while reducing oxygen to hydrogen peroxide. HAO1 prefenentially oxidizes the substrate glycolate and also oxidizes other substrates, including 2-hydroxy fatty acids as well as L-?hydroxy acids of moderately short chain lengths. The oxidation of glycolate yields glyoxylate which is utilized for peroxisomal synthesis of glycine. HAO1 is also able to convert glyoxylate to oxalate. HAO1 is thought to play a role in the pathophysiology of hyperoxaluria type 1, which is caused by defects in AGXT, a peroxisomal enzyme, leading to accumulation of glyoxylate. Hyperoxaluria type 1 is characterized by an accumulation of oxalate that is thought to lead to precipitates of calcium oxalate in kidneys which can be fatal. Function: Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. Subcellular Location: Peroxisome. Tissue Specificity: Liver. Similarity: Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain. SWISS: Q9WU19 Gene ID: 15112 Database links: Entrez Gene: 54363 Human Entrez Gene: 15112 Mouse Omim: 605023 Human SwissProt: Q9UJM8 Human SwissProt: Q9WU19 Mouse Unigene: 193640 Human Unigene: 26634 Mouse
Product Picture	Sample: Liver(Rat) Lysate at 40 ug Primary: Anti- HAO1 (SLM-33191M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 42 kD Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HAO1) Monoclonal Antibody, Unconjugated (ascites of SLM-33191M Mix) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.

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