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Product Name AmCyan Chinese Name AmCyan蓝色荧光蛋白单克隆抗体 Alias AmCyan Tag; AmCyan-Tag; 野生型天竺葵青色荧光蛋白; 蓝色荧光蛋白AmCyan标签; 蓝色荧光蛋白标签; Product Type Tag anti Research Area Cell biology Immunogen Species Mouse Clonality Monoclonal Clone NO. 5F9 React Species Recombinant protein Applications WB=1:1000-5000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Form Liquid Concentration 1mg/ml immunogen Recombinant AmCyan Lsotype IgG Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail AmCyan1 is a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (AmCyan) that exhibits enhanced emission characteristics (excitation and emission maxima: 458nm and 489nm, respectively). DD-AmCyan1 is a modified version of AmCyan1 that is tagged on its N-terminus with the ProteoTuner destabilization domain (DD). The presence of this destabilization domain causes rapid, proteasomal degradation of the fluorescent fusion protein; however, when the membrane permeant ligand Shield1 is added to the medium, it binds to the destabilization domain and protects the fusion protein from degradation.
SWISS:
N/A
Gene ID:
N/A
Product Picture Sample:
Lane 1: AmCyan-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: SL33184P) at 4 ug
Lane 2: Control (-) E.coli Lysate at 4 ug
Primary: Anti- AmCyan-Tag (SLM-33184M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
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