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Product Name TAP-Tag Chinese Name TAP-Tag(标签)单克隆抗体 Alias TAP Tag; TAPTag; Tandem affinity purification Product Type Tag anti Immunogen Species Mouse Clonality Monoclonal Clone NO. Mix-mA™ React Species Recombinant protein Applications WB=1:1000-5000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Form Liquid Concentration 1mg/ml immunogen Recombinant TAP-Tag Lsotype IgG Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Tandem affinity purification (TAP) is a purification technique for studying protein–protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix.
SWISS:
N/A
Gene ID:
N/A
Product Picture Sample:
Lane 1: TAP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 2ug
Lane 2: TAP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4ug
Primary: Anti-TAP-Tag (SLM-33133M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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