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Product Name Peroxiredoxin 1 Chinese Name 过氧化还原酶1单克隆抗体 Alias Peroxiredoxin-1; Peroxiredoxin 1; Prdx 1; Prdx1; PRDX1_HUMAN; Hbp23; Heme binding 23 kDa protein; Macrophage 23 Kd stress protein; Macrophage 23kDa stress protein; Macrophase stress protein 22kDa; Macrophase stress protein 23 kd; MSP23; Natural killer cell enhancing factor A; Natural killer cell-enhancing factor A; Natural killer enhancing factor A; NKEF A; NKEF-A; NkefA; OSF3; Osteoblast specific factor 3; PAG; Paga; PAGB; Proliferation associated gene A; Proliferation associated protein PAG; Proliferation-associated gene protein; PrxI; Tdpx 2; Tdpx2; TDX2; Thioredoxin dependent peroxide reductase 2; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2; TPxA; Trx dependent peroxide reductase 2. Research Area Tumour Cell biology immunology Immunogen Species Mouse Clonality Monoclonal Clone NO. 10F9 React Species Human, Mouse, Rat, Applications WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 22kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen Recombinant human Peroxiredoxin 1 Protein Lsotype IgG Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011].
Function:
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
Subunit:
Homodimer; disulfide-linked, upon oxidation. May form heterodimers with AOP2. Interacts with GDPD5; forms a mixed-disulfide with GDPD5 (By similarity).
Subcellular Location:
Cytoplasm. Melanosome.
Post-translational modifications:
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
Similarity:
Belongs to the AhpC/TSA family.
Contains 1 thioredoxin domain.
SWISS:
Q06830
Gene ID:
5052
Database links:
Entrez Gene: 5052 Human
Entrez Gene: 18477 Mouse
Omim: 176763 Human
SwissProt: Q9JKY1 Chinese Hamster
SwissProt: Q06830 Human
SwissProt: P35700 Mouse
Unigene: 180909 Human
Unigene: 30929 Mouse
Unigene: 2845 Rat
Product Picture Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Rat Spleen tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Human Jurkat cell lysates
Lane 7: Human Raji cell lysates
Lane 8: Human MCF-7 cell lysates
Lane 9: Human A431 cell lysates
Lane 10: Human Hela cell lysates
Primary: Anti-Peroxiredoxin 1 (SLM-33118M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 23 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peroxiredoxin 1) Monoclonal Antibody, Unconjugated (ascites of SLM-33118M 10F9) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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