Product Name	OCT-1
Chinese Name	胚胎Stem cells关键蛋白1单克隆抗体
Alias	class 2; transcription factor 1; FLJ42836; NF A1; NF-A1; Oct1; Oct-1; OCT1; Octamer binding protein 1; Octamer binding transcription factor 1; Octamer-binding protein 1; Octamer-binding transcription factor 1; OTF 1; OTF-1; OTF1; OTTHUMP00000032348; OTTHUMP00000032350; OTTHUMP00000032351; PO21; PO2F1; PO2F1_HUMAN; POU class 2 homeobox 1; POU domain; POU domain class 2 transcription factor 1; POU2F1.
Research Area	Tumour  Cell biology  immunology  Stem cells  transcriptional regulatory factor
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	9A3
React Species	Human,
Applications	WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	76kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Oct1
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes. Function: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes. Subunit: Interacts with NR3C1, AR, PGR and HCFC1. Associates with the herpes simplex virus VP16-induced complex; binding to HCFC1 activates the viral transcriptional activator VP16 for association with POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous. Isoform 2 is lymphocyte-specific. Post-translational modifications: Phosphorylated by PRKDC. Similarity: Belongs to the POU transcription factor family. Class-2 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. SWISS: P14859 Gene ID: 5451 Database links: Entrez Gene: 5451 Human Entrez Gene: 18986 Mouse Entrez Gene: 171068 Rat Omim: 164175 Human SwissProt: P14859 Human SwissProt: P25425 Mouse SwissProt: P31503 Rat Unigene: 283402 Human Unigene: 245261 Mouse Unigene: 46306 Rat
Product Picture	Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-OCT-1 (SLM-33095M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 80 kD Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-OCT-1 (SLM-33095M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 80 kD Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OCT-1) Polyclonal Antibody, Unconjugated (SLM-33095M) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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