Product Name	beta I Tubulin (Loading Control)
Chinese Name	微管蛋白β1 tubulin(内参)单克隆抗体
Alias	2810484G07Rik; Beta tubulin 1, class VI; Class VI beta tubulin; dJ543J19.4; M(beta)1; TBB1_HUMAN; TUBB1; Tubulin beta 1 class VI; Tubulin beta-1 chain; Tubulin, beta 1; tubulin, beta1;
literatures	Specific References  (1)     |     SLM-33041M has been referenced in 1 publications. [IF=3.231] Ruihua Xu. et al. Exosomes Derived from Yak Follicular Fluid Increase 2-Hydroxyestradiol Secretion by Activating Autophagy in Cumulus Cells. ANIMALS. 2022 Jan;12(22):3174  IF ;  Bovine.   PubMed:36428401
Product Type	Internal reference anti
Research Area	Cell biology  immunology  Signal transduction  Cytoskeleton
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	5F7
React Species	Human, Mouse, Rat,
Applications	WB=1:5000-20000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human beta I Tubulin
Lsotype	IgG2a
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.[provided by RefSeq, Jul 2010] Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). Subunit: Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with RANBP10. Subcellular Location: Cytoplasm, cytoskeleton Tissue Specificity: Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins. Post-translational modifications: Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. DISEASE: Macrothrombocytopenia, autosomal dominant, TUBB1-related (MAD-TUBB1). The disease is caused by mutations affecting the gene represented in this entry. A congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets. Similarity: Belongs to the tubulin family. SWISS: Q9H4B7 Gene ID: 81027 Database links: Entrez Gene: 396427 Chicken Entrez Gene: 541271 Cow Entrez Gene: 485950 Dog Entrez Gene: 101836899 Hamster Entrez Gene: 81027 Human Entrez Gene: 545486 Mouse Entrez Gene: 679312 Rat Omim: 612901 Human SwissProt: P09203 Chicken SwissProt: Q9H4B7 Human SwissProt: A2AQ07 Mouse Unigene: 303023 Human Unigene: 45285 Mouse
Product Picture	Sample: K562 Cell (Human) Lysate at 40 ug Jurkat Cell (Human) Lysate at 40 ug Primary: Anti- beta I Tubulin (SLM-33041M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti- beta I Tubulin (SLM-33041M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD Sample: 293T(Human) Cell Lysate at 30 ug Cerebellum (Mouse) Lysate at 40 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti- beta I Tubulin (SLM-33041M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: Cerebrum (Rat) Lysate at 40 ug Lane 3: Cerebrum (Mouse) Lysate at 40 ug Lane 4: Fetal brain (Mouse) Lysate at 40 ug Lane 5: Heart (Mouse) Lysate at 40 ug Lane 6: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 7: Spleen (Rat) Lysate at 40 ug Lane 8: Spleen (Mouse) Lysate at 40 ug Lane 9: Ovary (Rat) Lysate at 40 ug Primary: Anti-beta I Tubulin (SLM-33041M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (ascites of SLM-33041M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (ascites of SLM-33041M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (SLM-33041M 5F7) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (SLM-33041M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining. Tissue/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (SLM-33041M) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0296G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.

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