Product Name	alpha Tubulin
Chinese Name	微管蛋白α/Tubulin α/α-tubulin单克隆抗体
Alias	Alpha tubulin 1; Alpha-tubulin 1; Detyrosinated alpha Tubulin; FLJ30169; H2 alpha; TBA4A_HUMAN; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA 4A; TUBA1; Tuba4a; Tubulin alpha 1 (testis specific); Tubulin alpha 1; Tubulin alpha 1 chain; Tubulin alpha 4a; Tubulin alpha 4A chain; Tubulin alpha-1 chain; Tubulin alpha-4A chain; Tubulin H2 alpha; Tubulin H2-alpha; TUBA4A; α-tubulin; α tubulin; Tubulin-alpha.  α tubulin;
literatures	Specific References  (3)     |     SLM-33039M has been referenced in 3 publications. [IF=6.513] Jinglei Wang. et al. Follicular fluid exosomes regulate OVGP1 secretion in yak oviduct epithelial cells via autophagy in vitro. J CELL PHYSIOL. 2023 Apr;:  IF ;  Bovine.   PubMed:37013674 [IF=3.895] Shen X et al.Retinoic Acid-Induced Protein 14 (RAI14) Promotes mTOR-Mediated Inflammation Under Inflammatory Stress and Chemical Hypoxia in a U87 Glioblastoma Cell Line.(2018) Cell Mol Neurobiol.  WB ;  Human.   PubMed:30554401 [IF=3.471] Yaying Wang. et al. Transcriptome sequencing reveals differences between leydig cells and sertoli cells of yak.. FRONT VET SCI. 2022 Aug;9:960250-960250  IF ;  Bovine.   PubMed:36090173
Research Area	Cell biology  Signal transduction  Cytoskeleton
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	10F11
React Species	Human, Mouse, Rat, Zebrafish,  (predicted: Chicken, Dog, Pig, Rabbit, Guinea Pig, )
Applications	WB=1:50000-200000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human alpha Tubulin Protein
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain Subunit: Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Subcellular Location: Cytoplasm, cytoskeleton Tissue Specificity: Widely expressed. Overexpressed in Platelet, Brain, and Frontal cortex Post-translational modifications: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable). Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling (By similarity). Similarity: Belongs to the tubulin family. SWISS: P68366 Gene ID: 7277 Database links: Entrez Gene: 7277 Human Entrez Gene: 22145 Mouse Entrez Gene: 316531 Rat Omim: 191110 Human SwissProt: P68366 Human SwissProt: P68368 Mouse SwissProt: Q5XIF6 Rat Unigene: 75318 Human Unigene: 1155 Mouse Unigene: 92961 Rat
Product Picture	Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse NIH/3T3 cell lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Human HeLa cell lysates Lane 5: Human Jurkat cell lysates Lane 6: Human A431 cell lysates Lane 7: Human U251 cell lysates Lane 8: Human 293T cell lysates Lane 9: Human HepG2 cell lysates Primary: Anti-alpha Tubulin (SLM-33039M) at 1/100000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha Tubulin) Monoclonal Antibody, Unconjugated (ascites of SLM-33039M 10F11 ) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.

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