Product Name	Beta tubulin (Loading Control)
Chinese Name	微管蛋白β tubulin/Tubulin β（内参）单克隆抗体
Alias	Beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; Beta1 tubulin; Class I beta tubulin; M40; MGC117247; MGC16435; OK/SW cl.56; OK/SWcl.56; TBB5_HUMAN; TUBB 1; TUBB 2; TUBB 5; TUBB; TUBB1; TUBB2; TUBB5; tubulin beta 1 chain; Tubulin beta 2 chain; tubulin beta 5 chain; Tubulin beta chain; Tubulin beta class I; tubulin beta polypeptide; Tubulin beta-5 chain.
literatures	Specific References  (8)     |     SLM-33034M has been referenced in 8 publications. [IF=11.556] Yan Wang. et al. Sult2b1 deficiency exacerbates ischemic stroke by promoting pro-inflammatory macrophage polarization in mice. Theranostics. 2021; 11(20): 10074–10090  WB ;  Mouse.   PubMed:34815805 [IF=9.685] Jia, Xiuqin. et al. Inhibition of autophagy potentiates the cytotoxicity of the irreversible FGFR1-4 inhibitor FIIN-2 on lung adenocarcinoma. CELL DEATH DIS. 2022 Aug;13(8):1-12  WB ;  Human.   PubMed:36042213 [IF=7.077] Xinrui Li. et al. Skeletal muscle mass, meat quality and antioxidant status in growing lambs supplemented with guanidinoacetic acid. MEAT SCI. 2022 Oct;192:108906  WB ;  Sheep.   PubMed:35850029 [IF=6.656] Ying Yang. et al. Formononetin improves cardiac function and depressive behaviours in myocardial infarction with depression by targeting GSK-3β to regulate macrophage/microglial polarization. PHYTOMEDICINE. 2022 Dec;:154602  WB ;  Mouse.   PubMed:36610138 [IF=5.818] Hehe Du. et al. Cromileptes altivelis microRNA Transcriptome Analysis upon Nervous Necrosis Virus (NNV) Infection and the Effect of cal-miR-155 on Cells Apoptosis and Virus Replication. VIRUSES-BASEL. 2022 Oct;14(10):2184  WB ;  Cromileptes altivelis.   PubMed:36298739 [IF=3.644] Qin X et al. Effects of dietary sea buckthorn pomace supplementation on skeletal muscle mass and meat quality in lambs. Meat Sci . 2020 Aug;166:108141.  WB ;  lamb.   PubMed:32302933 [IF=2.985] Cao Y et al. Protective effect of carnosine on hydrogen peroxide–induced oxidative stress in Human kidney tubular epithelial cellsBiochem Biophys Res Commun.2021 Jan 1;534:576-582.  WB ;  Human.   PubMed:33276949 [IF=2.012] Yan Wang. et al. Telmisartan attenuates human glioblastoma cells proliferation and oncogenicity by inducing the lipid oxidation. 2021 May 04  WB ;  Human.   PubMed:33945216
Product Type	Internal reference anti
Research Area	Cytoskeleton  Extracellular matrix
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	7G3
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, Monkey, )
Applications	WB=1:5000-20000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:200-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Beta tubulin
Lsotype	IgG1
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014] Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Subunit: Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO. Interacts with MX1. Subcellular Location: Cytoplasmic, cytoskeleton. Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Post-translational modifications: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable). Similarity: Belongs to the tubulin family. SWISS: P07437 Gene ID: 203068 Database links: Entrez Gene: 203068 Human Entrez Gene: 22154 Mouse Entrez Gene: 29214 Rat Omim: 191130 Human SwissProt: P07437 Human SwissProt: P99024 Mouse SwissProt: P69897 Rat Unigene: 636480 Human Unigene: 2458 Rat
Product Picture	Sample: U87MG Cell (Human) Lysate at 40 ug SW480 Cell (Human) Lysate at 40 ug Primary: Anti- Beta tubulin (SLM-33034M) at 1/2 000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Sample: HL60(Human) Cell Lysate at 30 ug Primary: Anti- Beta tubulin (SLM-33034M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 52 kD Sample: HL60(Human) Cell Lysate at 30 ug U937(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug SH-SY5Y(Human) Cell Lysate at 30 ug Primary: Anti- Beta tubulin (SLM-33034M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Sample: A431(Human) Cell Lysate at 30 ug SY5Y(Human) Cell Lysate at 30 ug Primary: Anti- Beta tubulin (SLM-33034M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 52 kD Sample: Hela (Human) Lysate at 40 ug Primary: Anti-Beta tubulin (SLM-33034M) at 1/2000~1/20000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Beta tubulin (SLM-33034M) at 1/1000~20000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Beta tubulin (SLM-33034M) at 1/1000~1/20000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Sample: Hela(Human) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug HL60(Human) Cell Lysate at 30 ug SH-SY5Y(Human) Cell Lysate at 30 ug Cerebellum(Mouse) Lysate at 40 ug Cerebellum(Rat) Lysate at 40 ug Cerebrum(Mouse) Lysate at 40 ug Cerebrum(Rat) Lysate at 40 ug Primary: Anti-Beta tubulin (SLM-33034M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD Sample: A431(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Lovo(Human) Cell Lysate at 30 ug Primary: Anti- Beta tubulin (SLM-33034M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 52 kD Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (SLM-33034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (cell supernatant of SLM-33034M 7G3) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (cell supernatant of SLM-33034M 7G3) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (cell supernatant of SLM-33034M 7G3) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (SLM-33034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (SLM-33034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (ascites of SLM-33034M 7G3) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (ascites of SLM-33034M 7G3) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin) Monoclonal Antibody, Unconjugated (SLM-33034M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Beta tubulin) monoclonal Antibody, Unconjugated (SLM-33034M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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